226 Phylum Arthropoda 



Medium and containers should be changed once a week or oftener 

 to prevent the animals becoming entangled in bacteria. Calanus and 

 several of the other pelagic copepods may be kept for some days or even 

 weeks in a healthy condition in an icebox at 5°-6° C. if not too crowded, 

 and if kept in containers wide enough to allow an ample air supply. 



References 



Family Diaptomidae 



For the culture of Diaptomus see p. 143. 



Family cyclopidae 



CULTURE METHODS FOR CYCLOPOID COPEPODS 

 AND THEIR FORAGE ORGANISMS 



R. E. Coker, University of North Carolina 



Cyclops viridis, C. serrulatus, and C. vernalis have been reared very 

 successfully when fed on a mixed bacteria and protozoan culture with 

 occasional supplemental feeding from an algal culture; these cultures 

 are prepared by one of the methods described below. 



PROTOZOAN CULTURES 



Horse Manure. Fill several quart jars each % full of fresh horse 

 manure and nearly to the top with filtered tap water. After several days 

 add a few pipettes of pond water or some mixed protozoan culture to the 

 original jars; later jars are best inoculated from the best of the older 

 cultures. Putrid conditions existing during the first few days seem 

 effectively to sterilize the culture against copepods that might have been 

 introduced with the tap or pond water. Rich cultures of Protozoa should 

 develop within about a week; selection may then be made of the best 

 as measured by richness in Protozoa and bacteria and freedom from 

 cloudiness due to undesirable bacteria. A good jar may continue to 

 furnish a satisfactory food medium for 1 or 2 months, but fresh cultures 

 should be started at intervals of 2 or 3 weeks. 



Sheep Manure. Fill a jar % full of dried sheep manure (obtainable 

 from seed or fertilizer stores) and nearly to the top with water. Treat 

 like the horse manure cultures, although inoculation may not be 

 necessary. 



Strong hay infusion. This is prepared by steeping hay, preferably 

 timothy, in hot water for an hour or more (standard laboratory method) . 

 Place the strained liquid in open dishes and, after a few days, inoculate 

 as above. 



