Culicidae 3^3 



adjusted to maintain as closely as possible a temperature from 20 to 

 22 C. Close attention must be paid to the operation of incubators, as 

 mortality progressively increases as the temperature ascends above 22 C. 

 For their control they should be provided with maximum and minimum 

 thermometers which are read and set daily. 



Wooden rings are used as cages for the application of individual 

 infectious-mosquitoes' inoculation of patients. The rings have the same 

 diameter as the large cage frames and a small rim around the outer 

 edges so that the bobbinet squares may be retained by a rubber band. 

 One square is dyed black to facilitate observation of the mosquito. The 

 rings may be turned out of any dense wood. Their interior should be 

 sandpapered, painted white and sandpapered again to present a smooth 

 surface. A number of these rings, each with its mosquito, may be 

 placed in a copper petri dish can during transportation in the icebox. 



Overcrowding of the mosquitoes in a cage is an important cause of 

 injury. The risk increases with a rise in the temperature of storage. The 

 best results are secured when about 5 cubic inches of space is allowed for 

 each insect. The chilling of mosquitoes during transportation is very 

 important to immobilize them and reduce the danger from contusion 

 arising from collisions especially when they are full of blood. 



Success requires close and conscientious daily attention to the mos- 

 quitoes and in all handling and manipulation they should be treated with 

 all possible gentleness. 



Bibliography 

 Boyd, Mark F. 1926. A note on the rearing of anopheline larvae. Bull. Ent. Res. 



16:308. 

 IQ 30. The cage rearing of Anopheles quadrimaculatus. Amer. J. Trop. 



T 



Med. 9:165. 



A MOSQUITO REARING CAGE 



F. C. Baker, U. S. Bureau of Entomology and Plant Quarantine 



HIS indoor mosquito cage (Figs. 68 and 69) was constructed in the 

 spring of 1933 as a modification of the Boyd anopheline breeding 

 cage. A brief description of this cage is recorded, not because it is an 

 ideal type to be copied in detail, but for three other merits: (1) It was 

 fairly successful, both in the maintenance of a cage colony of Anopheles 

 quadrimaculatus and in culturing Aedes triseriatus and Culex pipiens. 

 (2) The cage and equipment cost only about $25. (3) It may help 

 someone else in designing his own artificial mosquito habitat to fit pre- 

 vailing circumstances when a greenhouse is available. 



The mosquito rearing cage is located in one of the compartments of 

 an insectary greenhouse. It is 4' * IO ' and "' hi S n - Its len S tn 

 extends in an east- west direction. On the south and west, 3' of its height 



