412 Phylum Arthropoda 



the paper covers once or twice daily the foliage within did not dry out 

 appreciably. 



This season I have been able to get females of Leschenaultia exul mated 

 in the laboratory and to produce fertile eggs. There is only one genera- 

 tion each year. The adults emerge from the overwintering puparia 

 (normally in the soil but held in the laboratory in moist sawdust or 

 peat moss) in April and May. The males emerge at least three days 

 earlier than the females. The gestation period was about two weeks 

 for the adults held this spring but it might be shorter normally since it 

 was an unusually cool spring in eastern Massachusetts where the work 

 was done. The reared females deposited eggs on foliage placed in the 

 containers just as the collected females did. Although I have not reared 

 adults from reared adults I have obtained fertile eggs as mentioned above 

 and have had host larvae parasitized by the maggots from such eggs. 



Family sarcophagidae 



A NEW TECHNIQUE FOR REARING DIPTEROUS LARVAE* 



IN rearing larvae of the Sarcophagidae and also of other Diptera such 

 as the Phoridae, Borboridae, etc., a nutritive medium commonly used 

 in bacteriological technique has been used with success. It consists of 10 

 parts of nutritive agar and i part of normal horse serum. This medium 

 has the great advantage of being sufficiently transparent to allow the 

 observation of all stages of development under a binocular microscope. 

 The medium may be kept in ampules of 50 cc, dated and labeled, and 

 thus may be kept for months, always ready for use. When eggs or 

 larvae of the larger species, or a fertilized female when one is dealing 

 with small species, are placed within the ampules or in other tubes with 

 the medium, the open end may be plugged with cotton or covered with a 

 fine piece of cloth which permits entrance of air. 



The puparia are formed on the surface of the medium or on the walls 

 of the tube, and generally one can see the segmentation in the adult under 

 the microscope by transmitted light. 



M. E. D. 



SARCOPHAGA BULLATA 



Roy Melvin, U. S. Bureau of Entomology and Plant Quarantine 



EIGHT generations of this species were reared at a temperature of 86° 

 to qo° F. during the winter of 1934-35. 

 The adults are handled in the same way as are those of Cochliomyia 



♦Slightly condensed from a translation by Julio Garcia-Diaz, University of Puerto 

 Pico, of an article by H. de Souza Lopes in Rev. de Entomologia 5:502, 1935. 



