Calliphoridae 4 J 7 



Several culture dishes (p) may be stacked in the same can by using 

 square wooden frames between (w). Food should be added as needed, 

 care being taken that no great amount of liquified material accumulates 

 in the dishes. As the larvae mature they creep out on the flat edges of 

 the plate (p) and fall to the damp earth (e) which fills the bottom of 

 the can to a depth of 7-10 cm. Here the larvae pupate. When the flies 

 emerge they creep up the walls of the can and into the cage. The damper 

 may then be closed; the cage may be removed, and the flies delivered 

 where desired. When all the flies have emerged, the can is ready for 

 immediate use again for the earth in the bottom may be used for a num- 

 ber of months without change. 



Much remains to be done to make the technique of blowfly culture 

 more precise. Conditions of temperature and humidity, oviposition 

 media, and larval foods all require additional investigation. A rather 

 detailed study of this sort has been made on Lucilia sericata by Cousin 

 (1929). Additional work is needed, however, on the requirements of 

 different species, and it is to be hoped that one result of the present 

 account may be the publication of useful experiences of other investi- 

 gators working with these forms. 



Bibliography 



Cousin, G. 1929. Sur les conditions indispensables a la nutrition et a la ponte de 

 Lucilia sericata Meig. C. R. Soc. Biol. 100:570. 



1929. L'alimentation de Lucilia sericata Meig. et ses relations avec la ponte. 



Ibid. 100:648. 

 — 1929. Sur le mode de ponte de Lucilia sericata Meig. Ibid. 100:731. 

 -1929. Sur les relations entre l'accouplement et le reflexe de ponte de Lucilia 



sericata Meig. Ibid. 100:818. 



-1929. Sur les conditions exterieures determinant le lieu de ponte de Lucilia 



sericata Meig. Ibid. 100:820. 



1929. Remarques sur la vie larvaire de Lucilia sericata Meig. Ibid. 101:653. 



Conditions externes necessaires pour obtenir un developement normal 



des larves de Lucilia sericata Meig. Ibid. 101:788. 



-1929. Influence de l'etat hygrometrique du mileu sur revolution larvaire de 



Lucilia sericata Meig. Ibid. 101:913. 



-1929. Influence de l'alimentation, des gaz toxiques et de la temperature sur la 



diapause de Lucilia sericata Meig. Ibid. 101:1115. 



-1929. Les facteurs externes qui determinent la reprise de revolution 



larvaire de Lucilia sericata Meig. et leur relation avec les influences exterieures 



qui out provoque la diapause. Ibid. 101:1117. 

 Haub, J. G., and Miller, D. F. 1932. Food requirements of blowfly cultures used 



in the treatment of osteomyelitis. /. Exper. Zool. 64:51. 

 Minnich, D. E. 1929. The chemical sensitivity of the legs of the blowfly, Calli- 



phora vomitoria Linn., to various sugars. Zeitschr. vergl. Physiol. 11: 1. 



