252 



STATE BOARD OF AGRICULTURE 



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Fig. 2. 







Bacilli of ropy 

 cream. 



Upon studyirg the 

 Tliere is no 



into threads of four, five or six. The ends are round 

 and the protoplasm homogeneous in all of its stages 

 of development. When forming in threads, it is im- 

 possible to distinguish the individual bacilli; they 

 frequeatly resemble a single long bacillus. 



Colony: The first appearance of the colony is 

 round with a well defined and evea> border, having a 

 blackish brown color atd finely granular contentB. 

 plate, the largest number of colonies will present this aspect, 

 liquefaction which 

 Chuses the colony to 

 sink into the gelatin, 

 but the surface col- A 

 onies rest on and 

 rise above it. Cun- 

 tinuing to develop, 

 the colony sends out 

 from its border a 

 thin and light color- 

 ed plaque wi th a bor- 

 der not so well de- 

 fined and somewhat 

 uneven, simply a 

 thin layer of micro- 

 organisms spread- ^'^- ^- Colonies of ropy cream bacillus, 

 ing from the maia bDdy of the colony over the surface of the gelatin 

 Again from this thin layer, as the colony grows older, another layer staits 

 thinner than the one from which it proceeds and is almost void of color, 

 translucent. The border is still less regular and almost ragged; some- 

 times it has a scalloped appearance. To the naked eye, the colony glistens 

 and gives a rounded oval surface. Upon the application of the platinum 

 needle, the colony will often adhere as a whole; at other times a ropy mass 

 of bacteria will be drawn out, From this will be inferred the tenacious 

 secretion of this bacillus. 



After cultivating this bacillus for some time and plating it in the same 

 gelatin, it was found that the colony had undergone a marked transforma- 

 tion, and in this state gave a loose patch work in its younger stage and a 

 very irregular colony as it matured. 



Gelatin Stick Culture: There is a slight growth along the line of inoc- 

 ulation, in some tubes simply a beaded development, in others quite a 



