264 STATE BOARD OF AGRICULTURE. 



and Bandeen made further bacteriological studies which gave additional 

 information relative to the microbial flora of the genital organs of cattle. 

 It will be noticed from table 1 that none of the cases examined showed 

 a great variety of organisms. In four cases the organisms obtained from 

 the surface of the mucous membrane were also obtained from the deeper 

 layers. In one case, B. coli commnnior was obtained from the deeper 

 layers and not from the surface of the mucous membrane. In another 

 case, B. coli communis was found in the pus of the right horn and not 

 any other place. Only in two cases did we obtain any organisms from 

 the tissues of the inoculated guinea pigs and those organisms were also 

 found by the direct culture method. The blood samples from the guinea 

 pigs were all negative to the complement fixation and agglutination tests 

 for Bad. abortus antibodies. 



CONCLUSIONS. 



The cases examined are not suflScient in number to warrant us. to 

 draw any definite conclusions. However, in view of the fact that all the 

 animals examined had aborted and came from herds where abortion 

 was prevalent at the time when these cows were slaughtered, one may 

 feel justified in considering the results obtained as additional evidence 

 that the Bad. ahortus does not persist indefinitely in the genital tract 

 and does not penetrate into the deeper layers of the mucous membrane 

 and remain there as a latent infection. 



REFERENCES. 



1. Cotton, W. E. 



1913. The persistence of the bacillus of infectious abortion in the 

 tissues of animals. 

 Am. Vet. Keview, Vol. 44, p. 307. 



2. Schroeder, E. C, and Cotton, W. E. 



191G. Some facts about abortion disease. 



Jour. Am. Vet. Med. Assn., Vol. 3, No. 3. 



3. Cotton, W. E. 



1919. Abortion disease of cattle. 



Jour. Am. Vet. Med. Assn., Vol 15, No. 5, Aug., p. 504. 



4. Giltner, Ward, and Bandeen, S. G. 



1920. The Bacteriology of the Reproductive Organs of the Cow 



and Its Relation to That of the Meconium of the Calf. 

 Journal, of Am. Vet. Med. Assn. 



5. Giltner, Ward. 



1917. Annual Report of Bacteriologist. 



Mich. State Board of Agr. Rept. p. 280. 



6. Hallman, E. T. 



1919. The Sequelae of Bact. Abortion Infection in the Bovine 

 Uterus. 

 Report of U. S. Live Stock Sanitary Association. 



7. Hallman, E. T. 



1919. A preliminary report on the pathology of the reproductive 

 organs in sterility. 

 Cornell Veterinarian, Jan., 1919. 



