266 



STATE BOARD OF AGRICULTURE. 



further simplify and facilitate the isolation and subsequent cultivation 

 of the abortion bacillus, much would be gained. With this point in mind 

 some experiments have been carried out in this laboratory during the 

 past six months with promising results. 



EXPERIMENTAL WORK. 



Employing the animal inoculation method as described by Smillie, it 

 was found that when the tubes were placed in an anaerobic jar and the 

 air was exhausted by a suction pump good growth was obtained in three 

 to four days. The employment of an anaerobic jar in this way is more 



Figure I. 



Fig. I shows the growth of a recently isolated strain on (1) plain agar, (4) spleen 

 starch agar and (8) plain liver agar after incubating for fifty-two hours. These cultures 

 were made from the first subculture obtained after the isolation of the strain. 



