EXPERIMENT STATION BULLETINS. 



267 



efficient and far more convenient than to seal each tube separately with 

 sealing wax. By placing the tubes next to the wall of the jar one can 

 quite easily detect growth without having to open the jar. The growth 

 obtained by this method was usually so abundant that heavy trans- 

 plants could be made, thus making it easier to get growth on the first 

 subculture. However, when transplants were made from the latter, the 



Figure II. 



Pig. II shows colonies of Bad. ahortus grown on plain liver agar. This plate is one 

 of eight plates made by making streaks with a platinum needle directly from the stomach 

 contents of a ftve-months-old fetus. On six plates pure cultures were obtained while two 

 showed slight contamination around the periphery. This plate had been incubated for 

 seven days when the picture was taken. The abundance of growth and the size of the 

 colonies show that such a long incubation would not have been necessary. 



