EXPERIMENT STATION BULLETINS. 329 



time of breeding the inferior abdominal surface of the bull should be dis- 

 infected and the sheath irrigated before and after service. In lieu of 

 antiseptics for uterine and vaginal injections we are using sour whey 

 obtained by curdling skim milk with the ordinary lactic acid organism 

 or with Bad. hulgaricum and find this to be a very effective remedy. 



Antiseptics suggested. For uterine and vaginal injections and for 

 flushing the sheath of the bull before and after service, a non-toxic, non- 

 irritating antiseptic should be used. For example, 1 to 1000 solution 

 of potassium permanganate, % per cent, solution of compound solution 

 of cresol or ^2 of 1 per cent, compound solution of iodine. Chinosol has 

 been strongly recommended as a powerful non-irritating antiseptic but 

 it is expensive. 



Potassium permanganate. A solution of potassium permanganate ap- 

 proximately 1 to 1000 may be conveniently made as follows: Place 2 to 

 4 ounces of potassium permanganate in a quart fruit jar and then fill 

 with water. Shake vigorously and allow all the undissolved crystals to 

 settle to the bottom.' The solution above the undissolved crystals is a 

 saturated solution. To dilute for using, take 4 teaspoonfuls of the 

 saturated solution to a quart of water or 2 ounces to one gallon of water. 

 The supply of saturated solution may be kept up by adding water and 

 sufficient potassium permanganate to keep more in the jar than will be 

 dissolved. Always let settle before using. 



Cresol. The cresols are very reliable but objectional in a dairy barn 

 on account of their disagreeable odor. A % per cent, solution of com- 

 pound solution of cresol can be approximated by adding 2 teaspooonfuls 

 of the compound solution of cresol to one quart of water, or 8 teaspoon- 

 fuls to one gallon of water. Clean water that has been boiled and cooled 

 should always be used in making up solutions. 



Sour luliey. The use of sour whey for uterine and vaginal injections 

 has already been referred to. We have been using sour whey for about 

 three years and have obtained better results from its use than from the 

 use of other antiseptics. The method of preparing this whey is as follows; 

 Place fresh skimmed milk in a clean vessel and add a culture of the 

 lactic acid organisms (cultures may be obtained from this laboratory at 

 25 cents each or creamery starter may be used if one is conveniently 

 located relative to a creamery). Set in a warm place (70-80° F.) until 

 a firm curd has fonned. Break this curd up into a number of small pieces 

 with a clean rod or spoon and filter through clean cheese cloth into 

 clean fruit jars. This whey is to be injected in about 4 to 8 ounce doses 

 daily into the uterus as long as it can be entered by ordinary methods 

 and 2 to 4 ounce doses into the vagina until discharges have ceased. 



Fresh whey should be made up at least once a week and kept in a cool 

 place until used. If too much acidity has developed it may be diluted 

 by adding water that has been boiled and cooled. Whether this is 

 needed may be determined by tasting. If irritating to the mouth it 

 should be diluted. 



In warm weather it is sometimes difficult to keep sour whey prepared 

 in this way longer than two or three days. Under such conditions the 

 following method of preparation is suggested: Bring fresh skimmed 

 milk to about 85° F. Add one dram of rennet in an ounce of clean 

 water to a gallon of this milk, stirring constantly while adding. Let 



