EXPERIMENT STATION BULLETINS. 



423 



TABLE III.— ACTIVE LARVAE OBSERVED. 



EXPERIMENT IV. 



January 26, 1914, dissected out a very large number of egg masses and 

 divided them into eight lots. After treatment each lot was transferred 

 to a plate of potato agar. 



1. Treated the first lot in 3% hydrogen peroxide for 10 minutes. 



2. Treated the second lot in 1-100 solution of 40% formaldehyde 

 for 10 minutes. 



3. Treated the third lot in 1-10 solution of 40% formaldehyde for 3 

 minutes. 



4. Treated the fourth lot in 40% formaldehyde for 10 minutes. 



5. Treated the fifth lot in 1-1000 HgCL for' 10 minutes. 



6. Treated the sixth lot in saturated solution of-HgCl, for a few 

 minutes. 



7. Treated the seventh lot in 20% copper sulphate for 30 minutes. 



8. Treated the eighth lot in carbon bisulphide vapor for ll^ hours. 



TABLE IV.— ACTIVE LARVAE OBSERVED. 



Treatment Jan. 26, 1914. 



Jan. 27. Jan. 28. Jan. 29. Jan. 30. Jan. 31. Feb. 1. Feb. 3. Feb. 4. Feb. 5, 



1. Peroxide 3% 



2. Formaldehyde 1-100, 



3. Formaldehyde 1-10. . 



4. Formaldehyde 40% . 



5. HgCl2 1-1000 



6. HgCl2Sat. Sol 



7. CuSO4 20% 



8. Carbon bisulphide . . 



15 



None 

 None 

 None 

 None 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 None 

 None 

 None 

 None 



Abund. 



None 

 None 

 None 

 None 

 None 

 None 

 None 



Abiind. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



Abund. 

 None 

 None 

 None 

 One 

 None 

 None 

 None 



EXPERIMENT V. 



February 2, 1914, dissected out a large number of egg masses from 

 knots on tomato roots and divided them into^ eight lots. After treat- 

 ment each lot was transferred to a plate of lima bean agar. 



1. Treated the first lot in 3% hydrogen peroxide for 30 minutes. 



2. Treated the second lot in 1-100 solution of 40% formaldehyde for 

 15 minutes. 



3. Treated the third lot in 1-10 solution of 40% formaldehyde for 15 

 minutes. 



4. Treated the fourth lot in 40% formaldehyde, full strength for 15 

 minutes. 



5. Treated the fifth lot in 1-1000 Hg CL for 15 minutes. 



6. Treated the sixth lot in a saturated solution of Hg CI2 for 30 

 minutes. 



7. Treated the seventh lot in 20% copper -sulphate for forty-five 

 minutes. 



