45G 



STATE BOARD OF AGRICULTURE. 



TABLE IV.— INFLUENCE OF CERTAIN INSECTICIDE AGENTS ON THE REDUCING 

 ACTIVITY (ON METHYLENE BLUE) OF EXTRACT FROM THE TISSUES OF PASS ALUS 

 CORN V TVS. 



(Solution of Meth. Blue =0.1 gram in 100 c.c. of dist. water.) 



o 

 IS 



6. 



X 



W 



1. 

 2. 

 3. 

 4. 

 6. 

 6. 

 7. 

 8. 

 9. 



10. 



11. 

 12. 



13. 



14. 



1.5. 

 16. 

 17. 



18. 

 19. 

 20. 



21. 

 22. 



23. 



24. 

 25. 

 26. 

 27. 

 28. 



29. 



30. 

 31. 



32. 

 33. 



34. 



Agent. 



Time extract 



was treated 



before being 



drawn into 



tube and 



sealed. 



Time required 

 I to reduce 

 Methylene 

 ; Blue in the 

 ! treated extract. 



Time required 



to reduce 



Methylene 



Blue in the 



check extract. 



Gasoline, 0.5 c.c. to 4 c.c. of the extract 



Gasoline, 1.0 c.c. m 3 c.c. of the extract 



Carbon disulphide, 0.5 c.c. in 3 c.c. of extract . . . 



Carbon disulphide, 0.5 c.c. in 3 c.c. of extract . . . 



Alcohol, 0.5 c.c. to 2 c.c. of extract 



Alcohol, 0.5 c.c. to 2 c.c. of extract 



Chloroform, 0.5 c.c. to 2 c.c. of extract 



Ether vapor, saturated 



Ether, 0.5 c.c. in contact with 2 c.c. of the ex- 

 tract 



Formaldehyde (40%) 3 drops in 2 c.c. of ex- 

 tract 



10 min 



3 min 



3 min 



23 min i 3 hrs 



5 min 6 min. 



15 min 10 min 



5 min 48 min 



35 min 5 min . 



35 min 



17 hrs 



1 hr., 16 min. . 

 21 min. 



min 17 min. 



7 min. 

 14 min. 

 14 min. 

 19 min. 

 1 J min. 

 2 min. 



8 min. 

 7 min. 



9 min. 



Tobakine. 1 drop to 2 c.c. of extract 



HCN.. (from KCN and H2SO4) strong charge 

 10 min. [aired 20 min.] 



Treated in a very strong Cyanide bottle, 2 hrs. 

 (aired 5 min.) 



Beetles treated for 15 hrs. in Cyanide bottle, 

 and then aired | hr. before extraction 



Toluol, 3 drops to 2 c.c. of extract 



Toluol (same as above) 



Pyrethrum, 0.5 c.c. dry powder to 2 c.c. of ex- 

 tract 



Pyrethrum (same as above) 



Pyrethrum (same as above) 



White Hellebore, 0.5 c.c. dry powder to 2 c.c. 

 extract , 



White Hellebore (same as above) ' 



White Hellebore (same as above) 



HgCU, 1 drop of saturated solution in 2 c.c. of; 

 extract 



NaF (sat. solution), 1 c.c. to 3 c.c. of extract; 



5 drops of Meth. Blue 



NaF (sat. solution), 1 c.c. to 3 c.c. of extract; 



5 drops of Meth. Blue 



NaF (sat. solution), 1 c.c. to 3 c.c. of extract; 



5 drops of Meth. Blue 



Borax (3 grms. in 100 c.c. water), 0.6 c.c. to 



2 c.c. of extract 



Borax (3 grms in 100 c.c. water), 0.6 c.c. to 



2 c.c. of extract 



M 

 Am. Formate, 1 c.c. of solution to 2 c.c 



5 min Still quite bluei 



after 24 hrs.. i 10 min. 

 5 min 10 min 1 10 min. 



10 min 30 min 



2 hrs 1 hr., 50 min. . 



18 min. 



4 min 51 min. 



15 min 30 min. 



5 min , 4 min. . 



27 min 38 min. 



2 hrs 18 hrs.. 



5 min 4 J min 



10 min 16j min 



97 min ....... Between 16 



I and 18 hrs. . 



5 min. 



8 min. . 

 25 min. 

 65 min. 

 10 min. 

 30 min. 



No apparent 

 reduction 

 at all 



1000 



of extract 



Temperature 58° C 



Temperature 0° F. for 3 hrs. 



6 min 



8 min 



21 min 



27 min. . . . . . 



1 hr., 50 min 



Temperature 70° F. stood for 3 hrs 



Carbon tetrachloride, 4 drops in 4 c.c. of ex- 

 tract 



Air saturated with vapor of carbon tetra- 

 chloride at 70° F 



i> min 15 min 



5 min About 10 hrs . 



After first 

 warming, 

 3^ min. 

 6 hrs 



15 min. 

 40 min. 



70 min. 

 3i hrs., 



4 min. 

 55 min. 



4 min. 

 2§ min. 

 15 min. 



1^ min. 



6 min. 



1 hr., 25 min. 



3 min. 



5 min. 



1 hr., 40 min. 



20 min. 

 3 min. 

 10 min. 

 1 hr., 50 min. 

 10 min. 

 46 min. 



5 min. 

 2 hrs. 

 Average of 

 fresh extract, 



1^ min. 

 1 min. 



18 min. 



li hrs. 



The results as given here are designed only to show whether the in- 

 fluence of the agent uijon the reducing power of the extract is marked 

 or not. The reducing activity of the tissue extract of P. CnrnuUis may be 

 entirely destroyed by a continuous temperature of 100° C. for ten to 

 fifteen minutes. While this high continuous temperature is necessary 

 for complete destruction, the greater part of the activity is quickly lost 



