478 



STATE BOARD OF AGRICULTURE. 



the iusects at the begiuning, the amount of gasoline vapor i)i'esent at 

 tlie start, and the amount absorbed, could be figured. 



At the end of the absorption test, the pupte were carefully 

 removed from the container and aired. They were then brought into the 

 laboratory, where they might warm up and start active development. 

 After their actions, taken in connection with respiration tests, showed 

 that they were in an "active state", an absorption test with gasoline- 

 vapor-air (using as nearly as possible the same percentage and the same 

 amount of gasoline vapor) was carried out in the warm room by the 

 same method as in the former case. 



Two separate experiments with Luna moth pupae, entailing the com- 

 plete sets of estimations just outlined, were carried out by this method. 

 Results of these two experiments are given in Table VIII, Nos. 1 and 2. 



TABLE VIII.— INSECTICIDE-VAPOR ABSORPTIONS OF COLD (DORMANT) AND WARM 



• (ACTIVE) INSECTS. 



Exp. 



No.l 



Insects. 



Vapor used. 



Temperature. 



: Volume of 

 I vapor absorbed. 



Time. 



5. 



, 6 Pupae of 

 9 Pupae of 



6 Pupae of Luna moth; 15 grms. . 



Pupae of 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 

 Passalus 



Luna moth; 

 Luna moth; 



Luna moth; 

 cornutus, 10 

 coniutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 11 

 cornutus, 11 

 cornutus, 11 

 cornutus, 11 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 

 cornutus, 10 



15 grras. . 

 20 grms . . 



20 grms . 



f,'rms Ether 



grms Ether 



grms Ether 



grms Ether 



Ether. 



Ether. 



Ether. 



Ether. 



Ether. 



Ether. 



Ether. 



Ether . 



Gasoline vapor 

 2.6% 



2.8% 



Gasoline vapor 



2.12% 



2.4% 



9.2° C I 0.38 c. c. 



24.6° C ' 1.13 c. c. 



.2 grms . . 

 .2 grms. . 

 .2 grras . . 

 .2 grms. . 

 .5 grms. . 

 .5 grms . . 

 .5 grms. . 

 5 grms . . 



grms Ether. 



grms Ether. 



grms Ether. 



grms Ether. 



7.2° C 



24.8° C 



. 6° C 



0.6° C 



22.2° C 



22.2° C 



-1.0° C 



-1.0° C 



23.4° C 



23.4° C 



-2.0° C 



-2.0° C 



24.0° C 



24.0° C 



-2.4° C 



About 20.0° C'. 

 About 20.0° C. 



0.35 c 

 0.89 c 

 1.2 c. 



1.4 c. 

 6.0 c. 



7.5 c. 

 2.40 c 

 2.45 <; 

 5.2 c. 



5.4 c. 

 0.7 c. 

 0.8 c. 

 5.1c. 

 6.0 c. 

 0.4 c. 

 0.5 c. 

 1.2 c. 



1.5 c. 



2 hrs. 

 2 hrs. 



1 hr. 



1 hr. 



10 min. 



.30 min . 



10 min. 



c j 30 min. 



c. . ; . . I 10 min. 



c I 15 min. 



c 10 rain. 



c 15 min. 



c 10 min. 



c 20 min . 



c 10 min. 



c 20 min . 



c 10 min. 



c 20 rain . 



c 10 min. 



c 20 min . 



At the beginning of Exp. Ko. 1, in the cold room, there were 2.08 c. c. 

 of gasoline vapor present in the air with the pupce. The table shows 

 that 0.38 c. c. of the vapor was absorbed — that is, only 0.18 of all the 

 gasoline vapor present at the start was absorbed by the six pupae. The 

 latter were all alive when brought into the warm room. They were kept 

 in moist air and their activity increased. By the third day, the respira- 

 tion had increased to more than three times its rate in the cold room. 

 On the sixth day, the rate was about the same or slightly less. The 

 pupae were kept in the warm loom 17 day.s, in all, before the last absorp- 

 tion test was made. Since, by the method used, not all the air was 

 expelled from the container before gasoline-air was drawn in with the 

 pupae above the mercury, it was impossible to make sure that the per- 

 centage of gasoline vapor used in the warm room was just the same as 

 was used in the cold room test. As may be seen by the table, however, 

 it was possible to make the percentages almost or practicalhj the same. 

 In the warm room test of Exp. 1, there were 2.24 c. c. of gasoline vapor 

 present in the air used, and 0..5-f of all this was absorbed by the same 



