EXPERIMENT STATION BULLETINS. 673 



APPENDIX. 



Media Used in Cultural Work with the Fungus. 



Cormneal Agar {Shear) : 



Four teaspoonfuls of yellow cornmeal were added to a liter of dis- 

 tilled water. Digested in water bath below 60° for one hour, 1.3% agar 

 was added and boiled for 45 minutes ; water lost added ; albumen of two 

 eggs added for clearing. Filtered through absorbent cotton and tubed. 

 Autoclaved 15 minutes 110° 



Prune Juice Agar: 



One hundred twenty gms. of dry prune flesh. Boiled in one liter of 

 distilled water in steamer for two hours. Filtered through cheesecloth 

 and 12 gms. of agar added. Boiled tW'O hours in steamer until com- 

 pletely dissolved. Cleared with albumen of two eggs. Filtered through 

 absorbent cotton. Autoclaved 15 minutes 110°. 



Tomato Leaf Agar: 



Green tomato leaves were pressed and 60 cc. of juice was boiled in 500 

 cc. of water and then 12 grams agar flour was added to juice, boiled 45 

 minutes and cleared with albumen of two eggs. Filtered through cot- 

 ton. Autoclaved 10 min. 110°. 



Nutrient Glucose Agar: 



Digested 20 gms. of agar in 500 cc. of water. Dissolved in 500 cc. 

 of water 40 gms. of glucose, 3 gms. of beef extract, 3 gms. of salt, 20 

 gms. of peptone. Poured into agar and steamed for 45 minutes. Cleared 

 with albumen of two eggs. Filtered through absorbent cotton. Auto- 

 claved 10 minutes at 110°. 



Nutrient Saccharose Agar: 



Digested 30 gms. of agar in 500 cc. of water. Dissolved in 500 cc. of 

 water -40 gms. of saccharose, 3 gms. beef extract, 3 gms, salt, 20 gms. 

 peptone. Poured into agar and steamed for 45 minutes. Cleared with 

 albumen of two eggs. Filtered through absorbent cotton. Autoclaved 

 10 minutes at 110°. 



Nutrient Broth: 



Dissolved 3 gms. of Leibig's Iteuf extract in a liter of water to which 

 was added 10 gms. peptone, 5 gms. of common salt; neutralized with nor- 

 mal NaOH to 1° Fuller's Scale. Autoclaved 10 minutes at 110°. 



Bean Pods: 



Canned bean pods placed with I/2 i"ch distilled water' in test tubes. 

 Autoclaved at 110° fifteen minutes. 



Potato Plugs: 



Potato plugs made with cork borer. Soaked in running water 24 

 hours. Placed in tubes in bottom of which a small wad of cotton soaked 

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