EXPERIMENT STA'JION REPORTS. 



1 Ao 



S.\]\rPLE XTX. 



hours after niilkilia:. Dilulioii 1-100. i;.° + . 



(lerms (in- CO. 



n hours aftor milkinjr. Dilution 1-10,000. 15° + . 



5°. 

 10°. 

 15°. 

 20°. 



20000 

 no colonies. 

 20000 

 10000 



30000 



40000 



'20000 



20000 



30000 

 (iOOOO 

 20000 

 20000 



30000 

 60000 

 20000 

 20000 



Milk from ten-gallon can at dairy barn. 



SAMPLE XX. 



hours after milking. Dilution 1-lCO. 13°-!-. 



.5°. 

 10°. 

 15°. 

 20°. 



19200 

 15700 

 23700 

 24000 



21540 

 21980 

 24400 

 24980 



22520 

 22520 

 25400 

 25000 



22700 

 23250 

 25600 

 25200 



22700 

 23450 

 25600 

 25200 



18700 

 21100 

 19900 

 18000 



hours after milking. Dilution 1-10,000. 13° + . 



no colonies. 

 10000 

 40000 

 30000 



Milk from ten-gallon can at dairy barn. 



Straight count on all plates of high dilution and good average counts on all others. 



40000 

 30000 

 30000 

 30000 



22500 

 25000 

 25200 

 25600 



40000 

 20000 

 50000 

 6Q000 



ACIDITY—FINAL SUMMARY. 



Temperature. 



21° 

 37° 



Per cent of Samples Growing Best at 



+ 5° 



5% 

 13% 



10° 



21% 



17% 



-1 15° 



64% 

 33% 



+ 20° 



10% 



17% 



DETERMINATION OF PER CENT LACTOSE. 



To the second lot of media was added the same percentage of agar 

 peptone, and salt as to Lot I. This was adjusted to + 15° * and 

 divided into five portions, to fonr of which were added 1%, 2%, 

 and 4% lactose, the fifth being left as ordinary agar. 



