EXPERIMENT STATION BULLETINS. 307 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 3 p. m. 

 23 hours after inoculation, Thursday, 2 p. m. 



lb — unchanged. 



lb + Ij — unchanged. 



Ij — unchanged. 

 41 hours after inoculation, Friday, 8 a. m. 



lb — no change. 



lb + Ij — litmus reduced. 



Ij — ^litmus reduced. 

 65 hours after inoculation, Saturday 8 a. m. 



lb — no change. 



lb + Ij — loppered. 



Ij — loppered. 



Acid developed during changes. 



Time after inoculation. hrs. 23 hrs. 41 hrs. 65 hrs. 



Culture lb 18° 18° 19° 19° 



Culture lb + Ij 18° 18° 28° 81° 



Culture Ij 18° 18° 30° 85° 



Bacterial Counts. 

 In 65 hours. 



Culture lb— below 1,000,000. 

 Culture lb + Ij— 3,200,000,000. 

 Culture Ij— 2,900,000,000. 



COMBINATION Ic AND Ij. 



Dilutions employed for inoculating milk flasks. 



Tnlture Ic 1 : 100,000,000 



Culture Ij 1 : 100,000,000 



Number of bacteria, Ic, introduced into milk flask Ic ^ 2. 

 Number of bacteria, Ij, introduced into milk flask Ij = 3. 

 Number of bacteria, Ic and Ij, introduced into milk flask Ic + Ij =2 + 3 = 5. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 3 p. m. 

 23 hours after inoculation, Tuesday, 2 p. m. 



Ic — unchanged. 



Ic + Ij — unchanged. 



Ij — unchanged. 

 47 hours after inoculation, Wednesday, 2. p. m. 



Ic — unchanged. 



Ic 4- Ij — litmus reduced near bottom. 



I j— litmus reduced near bottom. 

 71 hours after inoculation, Thursday, 2 p. m. 



Ic — unchanged. 



Ic + Ij — litmus reduced except surface. 



Ij — litmus reduced except surface. 

 114 hours after inoculation, Saturday, 9 a. m. 



Ic — unchanged . 



Ic + Ij — loppering slightly. 



Ij — more noticeable loppering. 

 138 hours after inoculation, Sunday, 9 a. m. 



Ic — unchanged. 



Ic + Ij — loppered. 



Ij — loppered more firmly. 



Acid developed during changes. 



Time after inoculation. hrs. 23 hrs. 47 hrs. 71 hrs. 114 hrs. 



Culture Ic 18° 18° 19° 20° 21° 



Culture Ic + Ij 18° 18° 22° 28° 39° 



Culture Ij 18° IS** 23° 31° 45° 



Bacterial counts. 

 In 71 hours. 



Culture Ic — No count. 

 Culture Ic +Ij— 202,000,000. 

 Culture Ij— 243,000,000. 



