30S STATE BOARD OF AGRICULTURE. 



COMBINATION Id AND Tj.* 



Dilutions employed for inoculating milk flasks. 



Culture Id 1 : 100 



Culture Ij 1 : 10,000 



Number of bacteria, Id, introduced into milk flask Id == 3,400. 

 Number of bacteria, Ij, introduced into milk flask Ij = 134,000. 

 Number of bacteria, Id and Ij, introduced into milk flask Id + Ij = 3,400 + 134 003 

 = 137,400. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 3 p. m. 

 24 hours after inoculation, Thursday, 3 p. m. 

 Id — unchanged. 



Id + Ij — litmus reduced at bottom only. 

 Ij — litmus reduced at bottom only. 



48 hours after inoculation, Friday, 3 p. m. 



Id — ^much the same as on Thursday. 

 Id + Ij — much the same as on Thursday. 

 Ij — much the same as on Thursday. 

 73 hours after inoculation, Saturday, 4 p. m. 

 Id — unchanged . 

 Id + Ij — ^litmus reddened. 

 Ij — litmus reddened. 



Acid developed during the changes. 



Time after inoculation. hrs. 24 hrs. 48 hrs. 73 hrs. 



Culture Id 18° 20° 21° 23° 



Culture Id + Ij 18° 25° 25° 27° 



Culture Ij 18° 23° 24° 27° 



Bacterial Counts. 



In 73 hours. 



Culture Id— 10,000,000. 

 Culture Id + Ij— 164,000,000. 

 Culture Ij— 117,000,000. 



COMBINATION le AND Ij. 



S Dilutions employed for inoculating milk flaskg. 



Culture le 1 : 1,000,000 



Cultiire Ij 1 : 100,000,000 



Number of bacteria, le, introduced into milk flask le = 348. 

 Number of bacteria, Ij, introduced into milk flask Ij = 14. 



Number of bacteria, le and Ij, introduced into milk flask le + Ij = 348 + 14 = 

 362. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Tuesday, 3 p. m. 



23 hours after inoculation, Wednesday, 2 p. m. 

 le — no change. 



le + Ij — litmus reduction almost complete. 

 Ij — litmus reduced at bottom only. 



49 hours after inoculation, Thursday, 4 p. m. 



le — ^no change. 



le + Ij — litmus wholly reduced. 

 Ij — litmus partly reduced. 

 72 hours after inoculation, Friday, 3 p. m. 

 le — litmus slightly changed. 

 le + Ij — beginning to curd at bottom. 

 Ij — ^litmus reddened. 

 Acid developed duriug changes. 



Time after inoculation. hrs. 23 hrs. 49 hrs. 72 hrs. 



• Culture le 18° 22° 25° 27° 



Culture le + Ij 18° 27° 35° 42° 



Culture Ij 18° 21° 25° 27 



o 



♦This test, which is the second, was interfered with because of the fact that the lactic micro- 

 organism had lost vigor from artificial cultivation. 



