310 STATE BOARD OF AGRICULTURE. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 3 p. m. 



24 hours after inoculation, Thursday, 3 p. m. 



Ig — unchanged. 



Ig + I j— litmus reduced at bottom and slightly reddened. 



Ij — unchanged. 



48 hours after inoculation, Friday, 3 p. m. 



Ig — litmus reduced, except near surface which is reddened. 



Ig + Ij — litmus reduced, except near surface which is reddened. 



Ij — litmus slightly reddened. 

 67 hours after inoculation, Saturday, 10 a. m. 



Ig — litmus wholly reduced. 



Ig + Ij — litmus wholly reduced. 



Ij — litmus reduced somewhat. 



Acid developed during changes. 



Time after inoculation. hrs. 24 hrs. 48 hrs. 67 hrs. 



Culture Ig 18° 22° 29? 30° 



Culture IJ + Ij 18° 33° - 39° 46° 



Culture Ij 18° 23° 26° 28° 



Bacterial Counts. 

 In 67 hours. 



Culture Ig— 511,000,000. 

 Culture Ig + Ij— 1,750,000,000. 

 Culture I j— 372,000,000. 



COMBINATION 111 AND Ij. 



Dilutions employed for inoculating milk flasks. 



Culture Ih 1 : 1,000,000 



Culture Ij 1 : 100,000,000 



Number of bacteria, Ih, introduced into milk fla^k Ih = 147. 



Number of bacteria, Ij, introduced into milk flask Ij == 22. 



Number of bacteria, Ih and Ij, introduced into milk flask Ih+ Ij = 147 + 22 = 163. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 3 p. m. 



25 hours after inoculation, Thursday, 4 p. m. 



Ih — no change. 



Ih + Ij — no change. 



Ij — no change. 

 41 hours after inoculation, Friday, 8 a. m. 



Ih — no change. 



Ih + Ij — litmus reduced. 



Ij — no change. 

 47f hours after inoculation, Friday, 2:15 p. m. 



Ih — no change. 



Ih + Ij — litmus red on surface, reduced below, loppered. 



Ij — no perceptible change. 



Acid developed during changes. 



Time after inoculation. hrs. 25 hrs. 41 hrs. 47 hrs. 



Culture Ih 16° 16° 16° 16° 



Culture Ih + Ij 16° 16° 32° 58° 



Culture Ij ., 16° 16 ° 16° 17 



Bacterial Counts. 

 In 47| hours. 



Culture 111-460,000,000. 

 Culture Ih + Ij— 2,400,000,000. 

 Culture Ij— 320,000,000. 



o 



