314 STATE BOARD OF AGRICULTURE. 



Bouillon. 



Turbid sediment at bottom with scum on surface. 

 Potato. 



Not noted. 

 Milk. 



Resembling lactic curd. See also chart combination Ig and Ij. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). Not noted. 

 Proteolysis. 



Gelatin liquefied. No proteolytic action on casein noted. 

 Biochemical tests. 



Hydrogen sulphide and reduction of nitrates, positive. Indol and ammonia, negative 

 Chromogenesis. 



No pigment. 



Ih. 



Morphology. Bacillus. 



Size — average diameter, .4 to .6 microns; length, .8 to 1 micron. 



Motility — motile. 



Staining — stains readily. 

 Gelatin Colony. 



Rovmd, fmely granular, raised. 

 Gelatin Stab. 



Saccate. 

 Inclined Agar. 



Dull, spreading, opalescent. 

 Bouillon. 



Turbid with sediment. 

 Potato. 



Moist abundant growth. 

 MUk. 



No change in seven days, eventually becoming curded. See also chart combina- 

 tion Ih and Ij. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). None noted. 

 Proteolysis. 



Liquefaction of gelatin; no proteolytic action on casein noted. 

 Biochemical tests. 



No production of indol, hydrogen sulphide, or ammonia. No reduction of nitrates. 

 Chromogenesis . 



No pigment produced. 



Ij. 



Morphology. (Micrococcus) Bacillus. 



Size — average diameter, .8 microns; length, about same as diameter 



Motility — no motility. 



Staining — stains readily. 

 Gelatin Colony. 



Round, reticulate. 

 Gelatin Stab. 



Filifonn. 

 Inclined Agar. 



Glistening, opalescent. 

 Bouillon. 



Turbid, some sediment. 

 Potato. 



Very slight growth. 

 Milk. 



Curded readily. See also chart combinations with Ij. 

 Fennentation of Sugars (Dextrose, Lactose, Saccharose). None. 

 Proteolysis. 



None. 

 Biochemical test. 



Indol, hydrogen sulphide, and ammonia, negative. Reduction of nitrates, negative. 

 Chromogenesis. 



No pigment produced. 



