EXPERIMENT STATION BULLETINS. 



Sir, 



SAMPLE II. 



The milk for this sample was taken from a ten gallon can placed in the 

 stable to receive the milk from the milkers. It was, therefore, as fresh as 

 could be secured from mixed milk and represented the product of several 

 cows. The milk may he said to be of a better quality than ordinary milk. 

 The sample thus obtained was taken to the laboratory in an Erlenmeyer 

 flask having a capacity of 375 c. cm. After reaching the laboratory the 

 acidity was determined and plates made. The milk was then placed at a 

 uniform temperature of 21° C. From time to time the acidity of the milk 

 was determined and plates made in order to follow the changes taking place 

 in the germ content. 



The methods followed in the study of this sample were identical with 

 those employed in Sample I. 



COMBINATION Ila AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Ila 1 : 10,000 



Culture IIx 1 : 1,000,000 



Niunber of bacteria, Ila, introduced into milk flask Ila = 540. 

 Number of bacteria, IIx, introduced into milk flask IIx = 2,000. 

 Number of bacteria, Ila and IIx, introduced into milk flask Ila + IIx 

 2,000 = 2,540. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Tuesday, 2 p. m. 



20 hours after inoculation, Wednesday, 10 a. m. 



Ila — unchanged. 



Ila + IIx — unchanged. 



IIx — unchanged . 

 48 hours after inoculation, Thursday, 2 p. m. 



Ila — tmchanged. 



Ila + IIx — unchanged. 



IIx — unchanged . 

 69 hours after inoculation, Friday, 11 a. m. 



Ila — unchanged. 



Ila 4- IIx — unchanged. 



IIx — unchanged. 

 93 hours after inoculation, Saturday, 11 a. m. 



Ila— unclianged . 



Ila + IIx — soft curd. 



IIx — soft curd. 

 Acid developed during changes. 

 Time after inoculation. 



Culture Ila 



Culture Ila + IIx... 

 Culture IIx. 



540 + 



