316 STATE BOARD OF AGRICULTURE. 



Bacterial Counts. 

 In 93 hours. 



Culture Ila— 102,000,000. 



Culture Ila + IIx— 1,990,000,000. 



Culture IIx— 1,980,000,000. 



COMBINATION lib AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture lib 1 : 10,000 



Culture IIx 1 : 100,000,000 



Number of bacteria, lib, introduced into milk flask lib = 17. 



Number of bacteria, IIx, introduced into milk flask IIx = 127,000. 



Number of bacteria, lib and IIx, introduced into milk flask lib + IIx == 17 + 

 127,000 = 127,017. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 3 p. m. 

 24 hours after inoculation, Friday, 3 p. m. 



lib — no change. 



lib + IIx — litmus slightly reduced. 



IIx — litmus slightly reduced. 

 43 hours after inoculation, Saturday, 10 a. m. 



lib — no change. 



lib + IIx — litmus reduced, no curd. 



IIx — litmus reduced, soft curd. 

 72 hours after inoculation, Sunday, 3 p. m. 



lib — no change. 



lib + IIx — litmus reduced, curded 



IIx — litmus reduced, curded. 



Acid developed during changes. 



Time after inoculation. hrs. 24 hrs. 43 hrs. 72 hrs. 



Culture lib 19° 20° 21° 23° 



Culture lib + IIx 19° 21° 49° 85° 



Culture IIx 19° 26° 58° 77° 



Bacterial Counts. 

 In 72 hours. 



Culture lib- 

 Culture lib + IIx— 3,220,000,000. 

 Culture IIx— 4,180,000,000. 



COMBINATION IIc AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture IIc 1 : 10,000 



Culture IIx 1 : 1,000,000 



Number of bacteria, IIc, introduced into milk flask IIc = 29,000. 

 Number of bacteria, IIx, introduced into milk flask IIx = 275,000. 

 Number of bacteria, IIc and IIx, introduced into milk flask IIc + IIx = 29,000 + 

 275,000 = 304,000. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 3 p. m. 

 24 hours after inoculation, Tuesday, 3 p. ni. 



IIc — no change. 



IIc + IIx=no change. 



IIx — no change. 

 48 hours after inoculation, Wednesday, 3 p. m. 



IIc — no change. 



IIc + IIx — litmus reduced; soft curd. 



IIx — litmus reduced; soft curd. 



Acid developed during changes. 



Time after inoculation. hrs. 24 hrs. 48 hrs. 



Culture IIc 22° 23° 23° 



Culture IIc + IIx 22° 23° 64° 



Culture IIx 22° 23° 62° 



