EXPERIMENT STATION BULLETINS. 



317 



Bacterial Counis. 

 In 48 hours. 



Culture lie— 442,000. 



Culture lie + IIx— 2,210,000,000. 



Culture IIx— 2,300,000,000. 



COMBINATION lid AND Ilx. 



Dilutions employed for inoculating milk flasks. 



Culture lid 1 : 1,000,000 



Culture llx 1 : 1,000,000 



Nmnber of bacteria, lid, introduced into milk flask lid = 17. 

 Number of bacteria, IIx, introduced into milk flask IIx = 1,800. 

 Number of bacteria, lid and IIx, introduced into milk flask lid + IIx 

 =1,817. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Tuesday, 3 p. m. 



24 hours after inoculation, Wednesday, 3 p. m. 



lid — no change. 



lid + IIx — no change. 



IIx — no change. 

 47 hours after inoculation, Thui'sday, 2 p. m. 



lid — no change. 



Ild + IIx — no change. 



IIx — no change. 

 71 hours after inoculation, Friday, 2 p. m. 



Ild — no change. 



Ild + IIx — litmus slightly reduced. 



IIx — litmus slightly reduced. 

 91 hours after inoculation, Saturday, 10 a. m. 



Ild — ho change. 



Ild + IIx — loppered. 



IIx — loppered. 



Acid developed during changes. 

 Time after inoculation. 



Culture lid 



Culture lid + IIx... 

 Culture IIx 



17 + 1,800 



Bacterial Counts. 

 In 91 hours. 



Culture lid— 1,330,000. 



Culture lid + IIx— 2,060,000,000. 



Culture IIx— 1,970,000,000. 



COMBINATION He AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture He 1 : 100 



Culture IIx 1 : 10,000 



Number of bacteria. He, mtroduced into milk flask He = 2,050,000. 

 Number of bacteria, IIx, introduced into milk flask IIx = 285,000. 

 Number of bacteria, He and IIx, introduced into milk flask He + IIx = 2,050.000 

 + 285,000 = 2,335,000. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 3 p. m. 

 23 hours after inoculation, Friday, 2 p. in. 



Ile^no change. 



He + IIx — no change. 



IIx — no change. 

 42 hours after inoculation, Saturday, 9 a. m. 



He — no change. 



He + IIx — loppering. 



IIx — litmus slightly reduced. 



