318 



STATE BOARD OF AGRICULTURE. 



Acid developed during changes. 



Time after inoculation. hrs. 23 hrs. 42 hrs. 



Culture He 18° 19° 20° 



Culture He + Hx 18° 19° 56° 



Culture Hx 18° 19° 38° 



Bacterial Counts. 

 In 42 hours. 



Culture He— 745,000,000. 



Culture He + Hx— 2,080,000,000. 



Culture Hx— 1,210,000,000. 



COMBINATION Hg AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Hg 1 : 100 



Culture Hx 1 : 1,000,000 



Nmnber of bacteria, Hg, introduced into milk flask Hg = 525,000. 

 Nmnber of bacteria, Hx, introduced into milk flask IIx = 1,820. 

 Number of bacteria, Hg and IIx, introduced into milk flask Hg + IIx = 525,000 + 

 1,820 = 526,820. 



Changes in milk flasks apparent to eye. 



Milk flask ciiltures prepared Friday, 4 p. m. 



18 hours after inoculation, Saturday, 10 a. m. 



Hg — no change. 



Hg + Hx — no change. 



IIx — no change. 

 42 hours after inoculation, Sunday, 10 a. m. 



Hg — no change. 



Hg + IIx — litmus reduced and slimy. 



IIx — litmus slightly reddened. 

 66 hours after inoculation, Monday, 10 a. m. 



Hg — no change in color; slimy. 



Hg + IIx — slimy curd and litmus reduced. 



IIx — soft curd; litmus reduced. 



Acid developed during changes. 



Time after inoculation. hrs. 18 hrs. 42 hrs. 66 hrs. 



Culture Hg 21° 20° 20° 21° 



Culture Hg + Hx 21° 22° 36° 79° 



Culture IIx 12° 22° 31° 83° 



Bacterial Counts. 

 In 66 hours. 



Culture Hg— 106,000,000. 



Culture Hg + IIx— 1,700,000,000. 



Culture IIx— 1,440,000,000. 



COMBINATION Ilh AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Ilh 1 : 100. 



Culture IIx 1 : 100,000,000 



Nmnber of bacteria, Ilh, introduced into milk flask Ilh = 693,000. 

 Nimaber of bacteria, IIx, introduced into milk flask IIx = 1,880. 

 Number of bacteria, Ilh and IIx, introduced into milk flask Ilh + IIx = 693,000 

 + 1,880 = 694,880. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 2 p. m. 

 24 hours after inoculation, Friday, 2 p. m. 



^Ilh^ — litmus reduced. 

 I Ilh + IIx — litmus reduced. 



IIx — litmus reduced. 

 44 hours after inoculation, Saturday, 10 a. m. 

 Ilh — litmus reduced. 



Ilh + IIx — litmus reduced and loppered. ; 



IIx — ^litmus reduced and loppered. _.] 



