EXPERIMENT STATION BULLETINS. 319 



Acid developed during changes. 



Time after inoculation. hrs. 24 hrs. 44 hrs. 



Culture Ilh. 21° 25° 25° 



Culture Ilh + IIx 21° 24° 86° 



Culture IIx 21° 24° 84° 



Bacterial Counts. 

 In 44 hours. 



Culture Ilh — Record destroyed. 

 Culture Ilh + IIx — Record destroyed. 

 Culture IIx — Record destroyed. 



COMBIN.^TION Hi AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Hi 1 : 10,000 



Culture IIx 1 : 1,000,000 



Number of bacteria, Hi, introduced into milk flask Hi = 2,190. 

 Number of bacteria, IIx, introduced into milk flask IIx = 1,630. 

 Number of bacteria. Hi and IIx, introduced into milk flask Hi + IIx == 2,190 + 1,630 

 = 3,820. 



Chnnges in milk flasks apparent to eye. 



Milk flask cultures prepared Friday, 2 p.m. 



26 hours after inoculation, Saturday, 4 p. m. 



Hi — no change. 



Hi + IIx — no change. 



IIx — no change. 

 48 hours after inoculation, Sunday, 2 p. m. 



Hi — no change. 



Hi + IIx — some change in litmus. 



IIx — some change in litmus. 

 67 hours after inoculation, Monday, 9 a. m. 



Hi — no change. 



Hi + IIx — loppered. 



IIx — loppered. 



Add developed during changes. 



Time after inoculation. hrs. 26 hrs. 48 hrs. 67 hrs. 



Culture Hi 19° 20° 20° 19° 



Culture Hi + IIx 19° 20° 27° 82° 



Culture IIx 19° 19° 28° 78° 



Bacterial Counts. 

 In 67 hours. 



Culture Hi— 4,000,000. 



Culture Hi + IIx— 3,300,000,000. 



Culture IIx— 1,900,000,000. 



COMBINATION IIj AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture IIj 1 : 1,000,000 



. Culture IIx 1 ; 100,000,000 



Number of bacteria, IIj, introduced into milk flask IIj = 1,480. 

 Number of bacteria, IIx, introduced mto milk flask IIx = 17,300. 

 Number of bacteria, IIj and IIx, introduced into milk flask IIj + IIx = 1,480 + 

 17,300 = 18,780. 



Changes in milk flasks apparent to eye. 



Milk flask ciiltures prepared Monday 3 p. m. 



24 hours after inoculation, Tuesday, 3 p. m. 



IIj — no change. 



IIj + IIx — no change. 



IIx — no change. 

 48 hours after inoculation, Wednesday, 3 p. m. 



IIj — litmus slightly reduced. 



IIj + IIx — loppered. j 



IIx — beginning to lopper. . ' 



