320 STATE BOARD OF AGRICULTURE. 



Acid developed during changes. 



Time after inoculation. hre. 24 hrs. 48 hrs. 



Culture IIj 21 ° 23° 30° 



Culture IIj + IIx 21 ° 23° 66° 



Culture IIx 21° 21° 58° 



Bacterial Counts. 

 In 48 hours. 



Culture IIj— 225,000,000. 



Culture IIj + IIx— 2,400,000,000. 



Culture IIx— 1,600,000,000. 



COMBINATION Ilk AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Ilk 1 : 10,000 



Culture IIx 1 : 1,000,000 



Number of bacteria, Ilk, introduced into milk flask Ilk = 96,000. 

 Number of bacteria, IIx, introduced into milk flask IIx = 48,000. 

 Number of bacteria. Ilk and IIx, introduced into milk flasks Ilk + IIx = 96,000 4- 

 48,000 = 144,000. 

 Changes in milk flanks apparent to eye. 



Milk flask cultures prepared Thursday, 2 p. m. 

 24 hours after inoculation, Friday, 2 p. m. 

 Ilk — no change. 

 Ilk + IIx — litmus reduced. 

 IIx — litmus reduced. 

 44 hours after inoculation, Saturday, 10 a. m. 

 Ilk — no change. 



Ilk + IIx — litmus reduced, loppered. 

 IIx— litmus reduced, loppered. 



Acid developed during changes. 



Time after inoculation. hrs. 24 hrs. 44 hrs. 



Culture Ilk 21° 22° 23° 



Culture Ilk + IIx 21° 25° 83° 



Culture IIx 21° 25° 86° 



Bacterial Counts. 

 In 44 hours. 



Culture Ilk— 15,800,000. 



Culture Ilk + IIx— 4,890,000,000. 



Culture IIx— 5,850,000,000. 



COMBINATION Ilni AND IIx. 



Dilutions employed for inoculating milk flasks. 



Culture Ilm 1 : 10,000 



Culture IIx 1 : 100,000 



Number of bacteria, Ilm, introduced into milk flask Ilm = 4,460. 

 Number of bacteria, IIx, introduced into milk flask IIx = 296,000. 

 Number of bacteria, Ilm and IIx, introduced into milk flask Ilm + IIx = 4,460 + 

 296,000 = 300,460. 

 Changes in milk flasks apparent to eye. 



Milk flask cultures, prepared Thursday, 3 p. m. 

 20 hours after inoculation, Friday, 11 a. m. 

 Ilm — no change. 

 Ilm + IIx — no change. 

 IIx- — no change. 

 44 hours after inoculation, Saturday, 11 a. m. 



Ilm — no change. , 



Ilm + IIx — litmus reduced. 

 IIx — litmus reduced. 

 Acid developed during changes. 



Time after inoculation. hrs. 20 hrs. 44 hrs. 



Culture Ilm 19° 19° 20° 



Culture Ilm -f IIx 19° 22° 48° 



Culture IIx 19^ 19° 54° 



