EXPERIMENT STATION BULLETINS. 325 



Milk. 



Smooth acid curd. See combination IIj and IIx. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). Dextrose fermented. 

 Proteolysis. 



Gelatin liquefied. Casein, no action noted. , . 



Biochemical tests. 



Indol produced. Nitrates reduced. Hydrogen sulphide, and ammonia, negative. 

 Chromogenesis. 



No pigment. 



Ilk. 



Morphologj\ Micrococcus. 



Size — average diameter, .8 microns. 



Motility — no motion. 



Staining — stains readily. 

 Gelatin Colony. 



Round, gnimose. 

 Gelatin Stab. 



Filifonn, becoming craterifomi. 

 Inclined Agar. 



Glistening, opaque. 

 Bouillon. 



Turbid, with sediment. 

 Potato. 



Abundant, dry growth. 

 Milk. 



Curded in nine days. Also see chart combination Ilk and IIx. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). Negative. 

 Proteolysis. 



Gelatin liquefied. No change of casein noted. 

 Biocliemical tests. 



Indol, and hydrogen sulphide, negative. Nitrates not reduced. 

 Chromogenesis. 



No pigment. 



IIx. 



Morphology. Bacillus. 



Size — average diameter, .5 microns; length, .7 to .8 microns. 



Motility — no motion. 



Staining — stains readily. 

 Gelatin Colony. 



Round, finely granular. 

 Gelatin Stab. 



Filifonn . 

 Inclined Agar. 



Glistening, opaque, creamy white. 

 Bouillon. 



Turbid, becoming clear, sediment at bottom. 

 Potato. 



Abundant growtli. 

 Milk. 



Curded, acid. Sec also coml)inations with IIx. 

 Fermentation of Sugars (Dextrose, Lactose, Sacoliarose). Negative. 

 Proteolysis. 



Negative. 

 Biochemical tests. 



No indol, hydrogen sulphide, or ammonia noted. Nitrates not reduced. 

 Chromogenesis. 



No pigment. 



SAMPLE III. 



This sample was secured in the dairy and may be regarded as ordinary 

 milk. It was placed in a steriHzed flask and brouiilit lo the laboratory. 

 Immediately plates were made and the acidity determined. The milk was 

 then placed at a temperature of 21° C. The acidity was tested and plates 

 made as indicated in the following work. 



