326 



STATE BOARD OF AGRICULTURE. 



SAilPLE III. 



Hours 



after 

 milking. 







■ 7, 



:i2. 

 47, 



r^?,. 



71, 



Acidity. Total number 



of 



samples. 



of 

 bacteria. 



17° 

 17° 

 10° 

 19° 

 4.3° 

 03° 

 72° 



221 ,000 



1,780,000 



."i, 800, 000 



204,000,000 



1,633,000,000 



1,936,000,000 



8.54,000,000 



Relative number of different types. 



I 

 2 1.003 



2 1 





 

 

 

 





 36 

 

 

 

 

 



220 



1782 



100 



100 



'4<) 



18 



1 





 



] 



i 



i 

 













 

 

 

 

 

 





 

 

 



119 

 1939 



854 



COMBINATION Ilia AND IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture Illa 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Ilia, introduced into milk flask Ilia = 115. 

 Nvmiber of bacteria, lllx, introduced into milk flask IIIx = 2,240. 

 Number of bacteria, Illa and IIIx, introduced into milk flask Ilia + IIIx 

 2,240 = 2,355. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 10 a. m. 

 6 hours after inoculation, Wednesday, 4 p. m. 



Illa — no change. 



Ilia + IIIx — no change. 



IIIx — no change. 

 22 hours after inoculation, Thm-sday, S a. ni. 



Illa — no change. 



Ilia + IIIx — litmus lighter. 



IIIx — no change. 

 54 hours after inoculation, Friday, 4 p. m. 



Ilia — no change. 



Ilia + IIIx — litmus nearly reduced. 



IIIx — litmus slightly reduced. 

 70 hours after inoculation, Saturday, 8 a. m. 



Ilia — no change. 



Ilia + IIIx — firm curd. 



illx — firm curd. 

 Acid developed during changes. 

 Time after inoculation. 



Culture Ilia 



Culture Ilia -I- IIIx.. 

 Culture IIIx 



= 115 + 



Bacterial Counts. 

 In 54 hours. 



Culture Illa -157,000,000. 

 Culture Ilia -!- IIIx -857,000,000. 

 Culture IIIx— 194,000,000. 



COMBINATION Illb AND IIIx. 



Dilutions employed for inoculating milk flasks 



Culture lllb 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Niunber of bacteria, Illb, introduced into milk flask Illb == 

 Number of bacteria, IIIx, introduced into milk flask IIIx =- 

 Number of bacteria, Illb and IIIx, introduced into milk flask 

 + 2,660 = 10,330. 



7,670. 

 2,660. 

 Illb + IIIx 



7670, 



