EXPERIMENT STATION BULLETINS. 



327 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 9 a. m. 

 8 hours after inoculation, Thursday, 5 p. m. 



Illb — no change. 



Illb + IIIx — no change. 



IIIx- — no change. 

 23 liours after inoculation, Friday, 8 a. m. 



Illb — no change. 



Illb + IIIx — no change. 



IIIx — no change. 

 31 hours after inoculation, Friday, 4 p. m. 



Illb — no change. 



Illb + IIIx — no change. 



IIIx — no change. 

 47 hours after inoculation, Saturday, 8 a. m. 



lllb — ^no change. 



Illb + IIIx — litmus slightly reduced. 



IIIx — no change. 

 59 hours after inoculation, Saturday 8 p. m. 



Illb — no change. 



Illb 4- IIIx— soft curd. 



IIIx — no change. 



Acid developed during changes. 



Time after inoculation. hrs. 8 hrs. 23 hrs. 31 hi-s. 47 hrs. 



Culture Illb 14° 14° 14° 14° 14° 



Culture Illb + IIIx 14° 14° 14° 14° 17° 



Culture IIIx 14° 14° 15° 15° 18° 



Bacterial Counts. 

 In 59 hours. 



Culture Illb— 208,000,000. 



Culture Illb + IIIx— 2,500,000,000. 



Culture IIIx— 222,000,000. 



COMBINATION IIIc AND IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture IIIc 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, IIIc, introduced into milk flask IIIc = 55,800. 

 Number of bacteria, IIIx, introduced into milk flask IIIx = 2,030. 

 Number of bacteria, IIIc + IIIx, introduced into milk flask IIIc + IIIx 

 + 2,030 = 57,830. 

 Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 10 a. m. 

 7 hours after inoculation, Thursday, 5 p. m. 

 IIIc — no change. 

 IIIc + IIIx — no change. 

 IIIx — ^no change. 

 22 hours after inoculation, Friday, S a. m. 

 IIIc — no change. 

 IIIc + IIIx — no change. 

 IIIx — no change. 

 29 hours after inoculation, Friday, 3 p. rn. 

 IIIc — no change. 



IIIc + IIJx — litmus slightly redviced. 

 IIIx — no change. 

 45.^ hours after inoculation, Saturday, 7:30 a. m. 

 IIIc — no change. 

 IIIc + IIIx— finn ciH'd. 

 IIIx — soft curd. 

 Acid developed changes. 



Time after inoculation. hrs. 7 hrs. 22 hrs. 31 hrs. 



Culture IIIc 14° 14° 15° 16° 



Culture IIIc + IIIx.. .. 14° 14° 15° 18° 



Culture IIIx 14° 14° 15° 18° 



59 hrs. 

 14° 



71° 

 20° 



55,800 



45* hrs. 

 iG° 

 71° 

 61° 



