:WS STATE BOARD OP AGRICULTURE. 



Bacterial Counts. 

 In 31 liours. 



Culture IIIc— 82,30300,000. In 24 hour . 

 Culture IIIc + II Ix— 312,000.000. 

 Culture IIIx— 116,000,000. 



COMBINATION Ille AND IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture Ille 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Ille, introduced into milk flask Ille == 8,810. 

 Nvmiber of bacteria, IIIx, introduced into milk flask IIIx = 2,410. 

 Number of bacteria, Ille and IIIx, introduced into milk flask Ille + IIIx ^= 8,810 

 + 2,410 = 11,220. 

 Chatujefi in milk Jlasks apparent to eye. 



Milk flask cultures prepared Monday, 9 a. m. 



8 hours after inoculation, Monday, 5 p. m. 

 Ille — no change. 

 Ille + IIIx — no change. 

 IIIx — no change. 

 24 liours after inoculation, Tuesday, 9 a. ui. 

 Ille — no change. 

 Ille + IIIx — no change. 

 IIIx — no change. 

 31 liours after inoculation, Tuesday, 4 p. m. 

 Ille — no change. 

 Ille + IIIx — no change. 

 IIIx — no change. 

 47 hours after inoculation, Wednesday, 8 a. m. 

 Ille — no change. 



Ille + IIIx — litmus reduced; loppering. 

 IIIx — slightly loppered. 

 71 hours after inoculation, Thursdaj', 8 a. m. 

 Ille — no change. 

 Ille + IIIx — loppered. 

 IIIx — loppered. 



Acid developed during change,^. 



'J'inie after inoculation. hrs. 8 hrs. 24 lirs. 31 hrs. 47 hrs. 71 hrs. 



Chilturc life 19° 19° 1S° 19° 17° 18° 



Ciilturc Ille + IIIx 19° 19° 19° 19° .50° 87° 



Culture IIIx 19° 19° 19° 19° 01° 98° 



Bacterial Counts. 

 In 47 liours. 



Culture Ille— 1,030,000 (24 hour count). 

 Culture Ille + IIIx— 2,230,000,000. 

 Culture IIIx— 2,410,000,000. 



COMBINATION Illf .\ND IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture Illf. 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Illf, introduced into milk flask Illf = 100,000. 

 Number of bacteria, IIIx, introduced into milk flask IIIx = 18. 

 Number of bacteria, Illf and IIIx, introduced into milk flask Illf + IIIx = 160,000 

 + 18 = 160,018. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 9 a. m. 

 7 hours after inoculation, Monday, 4 p. ra. 

 Illf — no change. 

 Illf + IIIx — no change. 

 IIIx — no change. 

 24 hours after inoculation, Tuesday, 9 a. m. 

 Illf — no change. 

 Illf + IIIx — no change. 

 IIIx — no change. 



