EXPERIMENT STATION BULLETINS. 329 



31 hours after inoculation, Tuesday, 4 p. m. 



Illf — no change. 



Illf + IIIx — no change. 



IIIx — htmus slightly reduced. 

 48 hours after inoculation, Wednesday, 9 a. m. 



Illf — litmus changing to red. 



Illf + IIIx — litmus reduced. 



IIIx — htmus becoming red. 

 53 hours after inoculation, Wednesday, 2 p. m. 



Illf — litmus red. 



Illf + IIIx— soft curd. 



IIIx — soft curd. 



Acid developed during changes. 



Time after inoculation. hrs. 7 hrs. 24 lirs. 



Culture Illf -14° 14° 14° 



Culture Illf + IIIx 14° 14° 17° 



Culture IIIx 14° 14° 14° 



Bacterial Counts. 

 In 53 hours. 



Culture Illf— 2,130,000,000. 

 Culture Illf + IIIx— 5,800,000,000. 

 Culture IIIx— 1,100,000,000. 



COMBINATION Illg and IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture Illg 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Illg, introduced into milk flask Illg = 10.800. 

 Nmnber of bacteria, IIIx, introduced into milk flask IIIx = 2,350. 

 Nmuber of bacteria, Illg and IIIx, introduced into milk flask Illg + IIIx = 10,800 

 + 2,350 = 13,150. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 

 8 hours after inoculation, Monday, 4 p. m. 



Illg — no change. 



Illg + IIIx — no change. 



IIIx — no change. 

 24 hours after inoculation, Tuesday, 8 a. m. 



Illg — no change. 



Illg + IIIx — no, change. 



IIIx — no change. 

 33 hours after inoculation, Tuesday, 5 p. m. 



Illg— ropy. 



Illg + IIIx — htmus reduced; ropy. 



IIIx — litmus very shghtly reduced. 

 47i hours after inoculation, Wednesday, 7:30 a. m. 



Illg — litmus slightly reduced; ropy. 



Illg + IIIx — litmus reduced; slimy. 



IIIx — litmus reduced. 



Acid developed during changes. 

 Time after inoculation. 



Culture Illg 



Culture Illg + IIIx.. 

 Culture IIIx 



Bacterial Counts. 

 In 47 i hours. 



Culture Illg— 1,400,000,000. 

 Culture Illg + IIIx— 2,300,000,000. 

 Culture IIIx— 1 ,500,000,000. 

 42 



