330 STATE BOARD OF AGRICULTURE. 



COMBINATION Illh AND IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture mil 1 : 10,000 



Culture IIIx 1 : 1.000,000 



Number of bacteria, IIIli, introduced into milk flask Illh = 898. 

 Number of bacteria, IIIx, introduced into milk flask IIIx = 3,800. 

 Number of bacteria, Illh + IIIx, introduced into flask milk Illh + IIIx = 898 + 

 3,800 = 4,698. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 

 9 hours after inoculation, Monday, 5 p. m. 



Illh — unchanged. 



Illh 4- IIIx— unchanged. 



IIIx — unchanged . 

 24 hours after inoculation, Tuesday, S a. m. 



Illh — unchanged. 



Illh + IIIx — unchanged. 



IIIx — unchanged. 

 33 hours after inoculation, Tuesday, 5 p. m. 



Illh — litmus very slightly reduced. 



Illh + IIIx — litmus very slightly reduced. 



IIIx — no change. 

 48 hours after inoculation, Wednesday, 8 a. m. 



Illh — litmus slightly reduced. 



Illh + IIIx — litmus slightly reddened. 



IIIx — litmus slightly reddened. 

 54 hours after inoculation, Wednesday, 2 p. in. 



mil — litmus slightly reduced. 



Illh + IIIx — litmus wholly reduced. 



IIIx — red; loppering. 



Acid developed during changes. 



Time after inoculation. hrs. 9 hrs. 24 hrs. 33 hrs. 48 hrs. 54 lirs. 



Culture Illh 15° 15° 15° 15° 15° 14° 



Culture Illh + IIIx 15° 15° 15° 15° 26° 37° 



Culture IIIx 15° 15° 15° 15° 35° 52° 



Bacterial Counts. 

 In 54 hours. 



Culture mil— 127,000,000. 



Culture Illh + IIIx— 1,300,000,000. 



Culture IIIx— 1,700,000,000. 



COMBINATION Illi and IIIx. 



Dilutions employed for inoculating milk flasks. 



Culture Illi 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Illi, introduced into milk flask Illi = 134. 

 Number of bacteria, IIIx, introduced into milk flask IIIx = 3,070. 

 Number of bacteria, Illi and IIIx, introduced into milk flask Illi + IIIx = 134 + 

 3,070 = 3,204. 

 Changes in milk flasks apparent to eye. . 



Milk flask culture prepared, Monday, 8 a. in. 

 9 hours after inoculation, Monday, 5 p. m. 

 Illi — unchanged. 

 Illi + IIIx — unchanged. 

 IIIx — unchanged . 

 24 hours after inoculation, Tuesday, 8 a. m. 

 Illi — unchanged. 

 Illi + IIIx — unchanged. 

 IIIx— unchanged. 

 33 hours after inoculation, Tuesday, 5 p. in. 

 Illi — unchanged. 



Illi + IIIx — litmus sliglill}' reduced. 

 I IIx — unchanged . 



