i 15° 15° 15° 15° 15° 



lUi + IIIx. .. 15° 15° 16° 19° 64° 

 inx. 15° 15° 15° 19° 55° 



EXPERIMENT STATION BULLETINS. 331 



48 hours after inoculation, Wednesday, 8 a. m. 

 Illi — unchanged. 

 Illi + IIIx— soft curd. 

 IIIx — loppering. 



Acid developed during changes. 



Time after inoculation. hrs. 9 hrs. 24 hi-s. 33 hrs. 48 hrs. 



Culture II 



Culture I 



Culture 

 Bacterial Counts. 

 In 48 hours. 



Culture Illi— 17,100,000. 



Culture Illi + IIIx— 2,250,000,000. 



Culture IIIx— 1,070,000,000. 



COMBINATION IIIj AXD Illx. 



Dilutions employed for inoculating milk flasks. 



Culture IIIj 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, IIIj, introduced into milk flask IIIj = 420. 

 Number of bacteria, IIIx, introduced into milk flask IIIx = 2,110. 

 Number of bacteria, IIIj and IIIx, introduced into milk flask IIIj + IIIx = 420 + 

 2,110 = 2,530. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 

 9 hours after inoculation, Monday, 5 p. m. 



IIIj — unchanged . 



IIIj + IIIx — unchanged. 



IIIx — inichanged . 

 24 hours after inoculation, Tuesday, 8 a. m. 



IIIj — unchanged. 



IIIj + IIIx — unchanged. 



IIIx — unchanged . 

 33 hours after inoculation, Tuesday, 5 p. m. 



IIIj — litmus slightly reduced. 



IIIj + IIIx — litmus reduced. 



IIIx — no change. 

 48 hours after inoculation, Wednesday, 8 a. m. 



IIIj — litmus reduced. 



IIIj + IIIx — litmus reduced. 



IIIx — no change. 

 57 hours after inoculation, Wednesday 5 p. m. 



IIIj — litmus reddened. 



IIIj + IIIx — litmus reduced; on point of curdling. 



IIIx — no change. 



Acid developed during clianges. 

 Time after inocula- 

 tion Ins. 9 hrs. 24 hrs. 33 hrs. 38 hrs. 57 hrs. 72 hrs. 



Culture IIIj 21° 21° 21° 25° 26° 30° 55° 



Culture IIIj + 



IIIx 21° 21° 21° 26° 26° 43° 63° 



Culture IIIx ... . 21° 21° 21° 23° 21° 21° 21° 



Bacterial Counts. 

 In 57 hours. 



Culture IIIj— 346,000,000. 



Culture IIIj + IIIx— 1,910,000,000. 



Culture IIIx— 171,000,000. 



COMBINATION Illk AND IIIx. 



Dilutions employed for moculating milk flasks. 



Culture Illk 1 : 10,000 



Culture IIIx 1 : 1,000,000 



Number of bacteria, Illk, introduced into milk flask Illk == 78,800. 

 Number of bacteria, IIIx, introduced into milk flask ITIx =^ 2,310. 

 Number of bactrriy, lllk and IIIx, introduced into milk flask Illk + IIIx = 78,800 

 + 2,310 = 81,110. 



