332 



STATE BOARD OF AGRICULTURE. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 4 p. m. 

 16 hours after inoculation, Thursday, 8 a. m. 

 Illk — no change. 

 Illk + IIIx — no change. 

 IIIx — no change. 

 25 hours after inoculation, Thursday, 5 p. m. 

 Illk — no change. 

 Illk + IIIx — no change. 

 IIIx — no change. 

 40 hours after inoculation, Friday, 8 a. m. 

 Illk — litmus slightly reduced. 

 Illk + IIIx— soft curd. 

 IIIx — soft curd. 

 Acid developed during changes. 



Time after inoculation. hrs. 



Culture Illk... 18° 



Culture Illk + IIIx 18° 



Culture IIIx 18° 



Bacterial Counts. 

 In 40 hours. 



Culture Illk— 187,000,000. 



Culture Illk + IIIx— 2,270,000,000. 



Culture IIIx— 2,280,000,000. 



Culture. 



No. bacteria 

 introduced 

 in culture.'' 



115 

 2,355 

 2,240 



7,G70 



10,330 



2.663 



55,800 



57,830 



2,030 



8,810 



11 ,220 



2,410 



160,000 



160,018 



18 



10,800 



13,150 



2,350 



898 

 4,693 

 3,800 



134 

 3 , 204 

 3,070 



420 

 2,530 

 2,110 



78.800 



81 ,110 



2,310 



No. bacteria 

 when 

 changed. 3 



157,000,000 

 857,000,000 

 194,000,000 



208,000,000 



2,500,000,000 



222,000,000 



82,300,000a 

 312,000,000a 

 116,000,000 



1,030.000 

 2.230,000,000 

 2,410,000.000 



2.130,000.000 

 5,800,000.000 

 1.100,000.000 



1.400,000,000 

 2,300.000.000 

 1,500,000.000 



127,000,000 

 1.300.000,000 

 1,700.000.000 



17.100.000 

 2,250.000.000 

 1.070.000.000 



346,000,000 



1,910,000,000 



171.000.000 



187.000.000 

 2,270.000,000 

 2.280.000.000 



Acidity* 



when 

 changed . 



10° 

 37° 

 21 ° 



14° 

 71° 

 20° 



16°b 



71° 



61° 



17° 

 56° 

 61° 



44° 



61° 

 69° 



28° 

 61° 

 42° 



14° 

 37° 

 52° 



15° 

 64° 

 55° 



30° 



43° 

 21° 



23° 

 64°- 

 57° 



Culture appearance 

 when changed. 



no change. 



litmus nearly reduced. 



litmus slightly reduced. 



no change, 

 soft curd, 

 no change. 



no change, c 

 firm curd, 

 soft curd. 



no change. 



litmus reduced; loppering. 



slightly loppering. 



litmus red. 

 soft curd, 

 soft curd. 



litmus slightly reduced; ropy, 

 litmus reduced; slimy, 

 litmus reduced. 



litmus slightly reduced, 

 litmus wholly reduced, 

 red; loppering. 



unchanged. 

 soft curd, 

 loppering. 



litmus appearing red. 



litmus reduced; point of curd 



no change. 



litmus slightly reduced, 

 soft curd, 

 soft curd. 



(a, in 31 hrs.) (b. in 45^ hrs.) (c. in 45i hrs.) (d. in 24 hrs.), instead of 47 hrs. 



'Constant letter representing the lactic micro-organisms. 

 -Variable letters, a, b, c, etc., the associate micro-organisms. 

 •■•Counls and acidities made at the same time unless otlierwi.se designated. 



"The columniindicating the number of bacteria introduced refers to the total number introduced 

 into the flask containing 100 c. cm. of milU. 



