338 STATE BOARD OF AGRICULTURE. 



Bactei-ial Counts. 

 In 55 hours. 



Culture IVb— 479,000,000. 

 Culture IVb + IVx— 989,000,000. 

 Culture IVx— 1,460,000,000. 



COMBINATION IVc AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVc 1 : 10,000 



Culture IVx 1 : 1,000,000 



Nmnber of bacteria, IVc, introduced into milk flask IVc =No record. 

 Number of bacteria, IVx, introduced into milk flask IVx = No record. 

 Number of bacteriaj IVc and IVx, introduced into milk flask IVc + IV.x = No 

 record. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Thursday, 8 a. m. 

 hours after inoculation, Thursday, 5 p. iii. 



IVc — no change. 



IVc + IVx— no cliange. 



IVx — ^no change. 

 24 hours after inoculation, Friday, 8 a. m. 



IVc — no change. 



IVc + IVx — no change. 



IVx — no change. 

 33 hours after inoculation, Friday, 5 p. m. 



IVc — no change. 



IVc + IVx — litmus almost wholly reduced. 



IVx — litmus slightly reduced. 

 48 hours after inoculation, Saturday, 8 a. m. 



IVc — no change. 



IVc + IVx — litmus almost wholly reduced. 



IVx — litmus becoming red. 

 57 hours after inoculation, Saturday, 5 p. m. 



IVc — litmus slightly reduced. 



IVc + IVx — litmus becoming red. 



IVx— htmus reddened. 



Acid developed during changes. 



Time after inoculation. hrs. 9 hrs. 24 hrs. 33 hrs. 48 hrs. 57 lu-s. 



Culture IVc 19° 19° 21° 18° 31° 31° 



Culture IVc + IVx..... 19° 21° 22° 22° 27° 43° 



Culture IVx 19° 21° 21° 21° 27° 44° 



Bactericd Counts. 

 In 57 hours. 



Culture IVc— 5,040,000,000. 

 Culture IVc + IVx— 3,780,000,000. 

 Culture IVx— 1,500,000,000. 



COMBINATION IVd AND IVx. 



Dilutions employed for inoculating milk fl.osks. 



Cultm-c IVd 1 : 10,000 



CuKure IVx 1 : 1,000,000 



Number of l)a,cterJa, IVd, introduced into milk flask IVd = 13,700. 

 Number of bacteria, IVx, introduced into milk flask IVx = 1,430. 

 Number of bacteria, IVd and IVx, introduced into milk flask IVd + IVx = 13,700 

 + 1,430 = 15,130. 



Changes in milk flasks apparent to eye. _ ' ^ 



Milk flask cultures prepared Monday, 8 a. m. 

 9 hours after inoculation, Monday, 5 p. ni. 

 IVd— no change. 

 IVd + IVx — no change. 

 IVx — no change. 

 24 hours after inoculation, Tuesday, 8 a ui. 

 IVd — no change. 

 IVd + IVx — no change. 

 IVx — no change. 



