EXPERIMENT STATION BULLETINS. 339 



32 hours after inoculation, Tuesday, 4 p. m. 

 IVd — no change. 

 IVd + IVx — no change. 

 IVx — no change. 



48 hours after inoculation, Wednesday, 8 a. m. 



IVd — no change. 



IVd + IVx — no change. 



IVx — slight reduction of litmus. 

 72 hours after inoculation, Thursday, 8 a. m. 



IVd — no change. 



IVd + IVx — no change. 



IVx — litmus reddened. 

 Acid developed during chatifjes 

 Time after inoculation. 



Culture IVd 



Culture IVd + IVx. 

 Culture IVx 



Bacterial Counts. 

 Destroyed . 



COMBINATION IVc AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVe 1 : 10,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVe, introduced into milk flask IVe == 1. 

 Number of bacteria, IVx, introduced into milk flask IVx = 375. 

 Number of bacteria, IVe and IVx, introduced into milk flask IVe + IVx = 1 + 375 

 = 376. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 10 a. m. 

 7 hours after inoculation, Wednesday, 5 p. m. 



IVe — ^no change. 



IVe + IVx — no change. 



IVx — no change. 

 24 hours after inoculation, Thursday, 10 a. m. 



IVe — no change. 



IVe + IVx — no change. 



IVx — no change. 

 31 hours after inoculation, Thursday, 5 p. m. 



IVe — no change. 



IVe + IVx — no change. 



I^'x — no change. 



49 hours after inoculation, Fi-iday, 11 a. ni. 



IVe — no change. 



IVe + IVx — loppered. 



IVx — litmus changed. 



Acid developed during changes. 



COMBINATION IVf AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVf 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVf, introduced into milk flask IVf == 1,680. 

 Nmnber of bacteria, IVx, introduced into milk flask IVx = 4.50. 

 Number of bacteria, IVf and IVx, introduced into milk flask IVf + IVx == 1,680 + 

 450 = 2,130. 



