340 STATE BOARD OF AGRICULTURE. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 7:30 a. m. 

 9h hours after inoculation, Monday, 5 p. m. 



IVf — no change. 



IVf + IVx — no change. 



IVx — no change. 

 24| hours after inoculation, Tuesday, 8 a. m. 



IVf — no change. 



IVf + IVx — no change. 



IVx — no change. 

 32h hours after inoculation, Tuesday, 4 p. in. 



IVf — litmus slightly reduced. 



IVf + IVx — litmus slightly reduced. 



IVx — ^no change. 

 49| hours after inoculation, Wednesday, 9 a. m. 



IVf — litmus reddened. 



IVf + IVx — litmus reddened and milk loppered. 



IVx — litmus slightly reduced. 



Acid developed during changes. 

 Time after inoculation. 



Culture IVf 



Culture IVf + IVx.. . 

 Culture IVx 



Bacterial Counts. 

 In 32| hours. 



Culture IVf— 308,000,000. 

 Culture IVf + IVx— 357,000,000. 

 Culture IVx— 26,000,000. 



COMBINATION IVg AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVg 1 : 10,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVg, mtroduced into milk flask IVg = 3,270. 

 Nmnber of bacteria, IVx, introduced into milk flask IVx = 2,300. 

 Number of bacteria, IVg and IVx, introduced into milk flask IVg + IVx = 3,270 + 

 2,300 = 5,570. 

 Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 9 a. m. 

 S hours after inoculation, Monday, 5 p. m. 

 IVg — unchanged . 

 IVg + IVx — unchanged. 

 IVx — unchanged. 

 24 hours after inoculation, Tuesday, 9 a. m. 

 IVg — unchanged . 

 IVg + IVx — unchanged. 

 IVx — unchanged. 

 32 hours after inoculation, Tuesday, 5 p. m. 

 IVg — unchanged . 



IVg + IVx — litmus mostly reduced. 

 IVx — litmus slightly reduced. 

 47 hours after inoculation, Wednesday, 8 a. m. 

 IVg — unchanged. 

 IVg + IVx — loppered. 

 IVx — loppered. 



Acid developed during changes. 

 Time after inoculation. 



Culture IVg 



Culture IVg + IVx.. 

 Culture IVx 



Bacterial Counts. 

 In 47 hours. 



Culture IVg— 1,000,000,000. 

 Culture IVg + IVx— 3,000,000,000. 

 Culture IVx— 1,000,000,000. 



