EXPERIMENT STATION BULLETINS. 341 



COMBINATION IVh AND IVx. 



Dilutions employed for iuoculatiug milk flasks. 



Culture IVh 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVh, introduced into milk flask IVh = 1,520. 

 Number of bacteria, IVx, introduced into milk flask IVx = 338. 

 Number of bacteria, IVh and IVx, introduced into milk flask IVh + IVx = 1,520 + 

 338 = 1,858. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 

 8 hours after inoculation, Monday, 4 p. m. 



IVh — no change. 



IVh + IVx — no change. 



IVx — no change. 

 24 hours after inoculation, Tuesday, 8 a. m. 



IVh — no change. 



IVh + IVx — no change. 



IVx — ^no change. 

 32 hours after inoculation, Tuesday, 4 p. m. 



IVh — no change. 



IVh + IVx — no change. 



IVx — no change. 

 48 hours after inoculation, Wednesday, 8 a. m. 



IVh — no change 



IVh + IVx — litmus changing to red. 



IVx — litmus reddened. 

 56 hours after inoculation, Wednesday, 4 p. m. 



IVh — no change. 



IVh + IVx — -loppered. 



IVx — Utmus reddened. , 



Acid developed during cJianges. 



Time after inoculation. hrs. 8 hrs. 24 hrs. 32 hrs. 48 hrs. 56 hrs. 



Culture IVh 14° 14° 14° 14° 14° 15° 



Culture IVh + IVx 14° 14° 14° 15° 25° 56° 



Culture IVx 14° 14° 14° 15° 30° 42° 



Bacterial Counts. 

 In 56 hours. 



Culture IVh— 723.000,000. 

 Culture IVh + IVx— 968,000,000. 

 Culture IVx— 604,000,000. 



COMBINATION IVi AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVi 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVi, introduced into milk flask IVi = 74, 

 Nimiber of bacteria, IVx, introduced into milk flask IVx = 1,560. 

 Number of bacteria, IVi and IVx, introduced into milk flask IVi + IVx = 74 + 1,560 

 = 1,634. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 2 p. m. 

 18 hours after inoculation, Thursday, 8 a. m. 



IVi — unchanged. 



IVi + IVx == imchanged. 



IVx — unchanged. 

 27 hours after inoculation, Thursday, 5 p. m. 



IVi — unchanged . 



IVi + IVx — unchanged. 



IVx — unchanged. 

 43 hours after inoculation, Fritlay, U a. ni. 



IVi — unchanged. 



IVi + IVx— loppered. 



IVx — loppered. 



