342 STATE BOARD OF AGRICULTURE. 



Acid developed during changes. 



Time aftei* inoculation. lirs. 18 lirs. 27 hrs. 43 hrs. 



Culture IVi 18° 18° 18° 22° 



Culture IVi + IVx 18° 18° 18° G8° 



Culture IVx 18° 18° 18° 60° 



Bacterial Counts. 

 In 43 hours. 



Culture IVi— 306,000,000. 



Culture IVi + IVx— 1,200,000,000. 



Culture IVx— 1,000,000,000. 



COMBINATION IVj AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVj 1 : 1.000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVj, introduced into milk flask IVj = 592. 

 Number of bacteria, IVx, introduced into milk flask IVx = 610. 

 Number of bacteria, IVj and IVx, introduced into milk flask IVj + IVx = 592 + 

 610 = 1,202. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 9 a. m. 

 7 hours after inoculation, Wednesday, 5 p. m. 



IVj — no change. 



IVj + IVx — no change. 



IVx — no change. 

 24 hours after inoculation, Tliursdaj^, 9 a. m. 



IVj — no change. 



IVj + IVx — no change. 



IVx- — no change. 

 31 hours after inoculation, Thursday, 5 p. m. 



IVj — no change. 



IVj + IVx — litmus nearly wholly reduced. 



IVx — no change. 

 47 hours after inoculation, Friday, 8 a. m. 



IVj — no change. 



IVj + IVx— litmus wholly reduced. 



IVx — iio change. 

 52i hours after inoculation, Friday, 1:30 p. m. 



IVj — no change. 



IVj + IVx — slimy curd. 



IVx — ^litmus red. 



Acid developed during chan/jes. 



Time after inociilation. hrs. 7 hrs. 24 hrs. 31 hrs. 47 hrs. 52?; hrs. 



Culture IVj 15° 15° 14° 15° 15° 15° 



Culture IVj + IVx 15° 15° 15° 15° 36° 44° 



Culture Hx 15° 15° 16° 15° 17° 22° 



Bacterial Counts. 

 In 52 i hours. 



Culture IVj— 2,750,000,000. 

 Culture IVj + IVx— 2,350,000,000. 

 Culture IVx— 211,000,000. 



COAIBIXATION IVm AND IVx. 



Dilution^ employed for inoculating milk flasks. 



Culture l^'m. . 1 : 10,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IV'm, introduced into milk flask IVm = 74,700. 

 Number of bacteria, IVx, introduced into milk flask IVx = 2,280. 

 Number of bacteria, IVm and IVx, introduced into milk flask IVm + IVx = 74,700 

 + 2,280 = 76,980. 



