EXPERIMENT STATION BULLETINS. 343 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared ]\Ionday, 9 a. m. 



8 hours after inoculation, Monday, 5 p. ra. 



IVm — unchanged. 

 IVm + IVx — unchanged. 

 I Vx — unchanged . 

 24 hours after inoculation, Tuesday, 9 a. m. 

 IVm — unchanged. 

 IVm + IVx — unchanged. 

 I Vx — unchanged . 



32 hours after inoculation, Tuesday, 5 p. m. 



IVm — litmus slightly reddened. 



IVm + IVx — litmus slightly reddened. 



IVx — unchanged . 

 48 hours after inoculation, Wednesday, 9 a. m. 



IVm — htmus reddened. 



IVm + IVx — litmus reddened. 



IVx — imchanged. 

 56 hours after inoculation, AVednesday, 5 p. m. 



IVm — litmus slightly reddened. 



IVm + IVx — loppered. 



IVx — litmus slightly reduced. 

 Acid developed during changes. 



Time after inoculation. hrs. 8 hrs. 24 hr? 



Culture IVm 18° 18° 21° 



Culture IVm + IVx 18° 18° 20° 



Culture IVx 18° 18° 18° 



Baclerial Counts. 

 In 56 houre. 



Culture IVm— 2,300,000,000. 

 Culture IVm + IVx— 1,800,000,000. 

 Culture IVx— 360,000,000. 



COMBINATION IVn AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVn 1 : 10,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVn, introduced into milk flask IVn = 52,600. 

 Nimiber of bacteria, IVx, introduced into milk flask IVx == 1,520. 

 Nimiber of bacteria, IVn and IVx, introduced into milk flask IVn + IVx = 52,600 

 + 1520 = 54,120. 



Changes in milk flusks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 



9 hours after inoculation, Monday, 5 p. m. 



IVn — unchanged. 

 IVn + IVx — unchanged. 

 IVx — -unchanged . 

 24 hours after inoculation, Tuesday, 8 a. m. 

 IVn — unchanged . 

 IVn -f IVx — -unchanged. 

 IVx — unchanged. 



33 hours after inoculation, Tuesday, 5 p. m. 



IVn — litmus slightly reddened. 

 IVn + IVx — litmus reduced. 

 IVx — litmus slightly reduced. 

 48 hours after inoculation, Wednesday, 8 a. in. 

 IVn — litmus reddened. 

 IVn + IVx — loppered. 

 IVx — loppered. 



