344 



STATE BOARD OF AGRICULTURE. 



Acid developed during changes. 



Time after inoculation. hrs. 9 hrs. 24 hrs. 33 hrs. 



Culture I Vn 22° 25° 28° 30° 



Culture IVn + IVx... 22° 25° 28° 38° 



Culture IVx 22° 25° 28° 32° 



Bacterial Counts. 

 In 48 hours. 



Culture IVn— 295,000,000. 

 Culture IVn + IVx— 600,000,000. 

 Culture IVx— 



COMBINATION IVo AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVo 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVo, introduced into milk flask IVo = 2,860. 

 Number of bacteria, IVx, introduced into milk flask IVx = 3,490. 

 Number of bacteria, IVo and IVx, introduced into milk flask IVo + IVx 

 + 3,490 = 6,350. 

 Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 2 p. m. 

 18 hours after inoculation, Thursday, 8 a. m. 

 IVo — unchanged. 

 IVo + IVx — unchanged. 

 IVx — unchanged. 

 27 hours after inoculation, Thursday, 5 p. m. 

 I Vo — unchanged . 

 IVo + IVx — litmus slightly red. 

 I Vx — luichanged . 

 42 hours after inoculation, Friday, 8 a. m. 

 IVo — litmus slightly reddened. 

 IVo + Vx — loppered. 

 IVx — loppered. 



Acid developed during changes. 



Time after inoculation. hrs. 16 hrs. 27 hrs. 



Culture IVo 20° 20° 20° 



Culture IVo + IVx 20° 20° 22° 



Culture IVx 20° 20° 21° 



Bacterial Counts. 

 In 42 hours. 



Culture IVo— 450,000,000. 



Culture IVo + IVx— 5,090,000,000. 



Culture IVx— 1,830,000,000. 



COMBIN.\TION IVp AND IVx. 



Dilutions employed for inoculating milk flasks. 



Culture IVp 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Number of bacteria, IVp, introduced into milk flask IVp = 0. 

 Number of bacteria, IVx, introduced into milk flask IVx = 1,800. 

 Number of bacteria, IVp + IVx, introduced into milk flask IVp + H'x 

 1,800 = 1,806. 

 Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Monday, 8 a. m. 

 9 hours after inoculation, Monday, 5 p. m. 

 IVp — unchanged. 

 IVp + IVx — unchanged. 

 IVx — I mchanged . 

 24 hours after inoculation. Tuesday, S a. m. 

 IVp— litmus slightly reduced. 

 IVp 4- IVx — litmus slightly reduced. 

 I Vx — unchanged . 



48 hrs. 

 64° 



72° 



2,800 



42 hrs 

 33° 



78° 

 66° 



= 6 + 



