EXPERIMENT STATION BULLETINS. 345 



33 hours after inoculation, Tuesday, 5 p. m. 

 IVp — litmus reddened and curded. 

 IVp + IVx — litmus curded. 

 IVx — litmus slightly reddened. 



Acid developed during changes. 



Time after inoculatiim. hrs. 9 hrs. 24 hrs. 33 hrs. 



Culture IVp 19° 19° 27° 58° 



Culture IVp + IVx 19° 19° 26° 66° 



Culture IVx 19° 19° 23° 40° 



Bacterial Counts. 

 In 33 hours. 



Culture IVp— 1,340,000,000. 

 Culture IVp + IVx— 1,380,000,000. 

 Culture IVx— 160,000,000 at 24 hours. 



COMBIN.\TION IVq .\ND IV.X. 



Dilutions employed for inoculating milk flasks. 



Culture IVq 1 : 1,000,000 



Culture IVx 1 : 1,000,000 



Niunber of bacteria, IVq, introduced into milk flask IVq = 7. 

 Number of bacteria, IVx, introduced into milk flask IVx == 1,260. 

 Number of bacteria. IVq and IVx, introduced into milk flask IVq + IVx = 7 + 1,260 

 = 1,207. 



Changes in milk flasks apparent to eye. 



Milk flask cultures prepared Wednesday, 8 a. m. 

 9 hours after inoculation, Wednesday, 5 p. m. 



IVq — unchanged. 



IVq + IVx — unchanged. 



IVx — unchanged. 

 24 hours after inoculation, Thursday, 8 a. m. 



IVq — unchanged. 



IVq + IVx — unchanged. 



IVx — unchanged. 

 33 hours after inoculation, Thursday, 5 p. m. 



IVq — litmus slightly reduced. 



IVq + IVx — litmus nearly wholly reduced. 



IVx — htmus slightly reduced. 

 48 hours after inoculation, Friday, 8 a. m. 



IVq — hjppered. 



IVq + IVx — loppered. 



IVx — loppered. 



Acid developed during changes. 



Time after inoculation. hrs. 9 hrs. 24 hrs. 33 hrs. 48 hrs. 



Culture IVq 19° 19° 21° 30° 96° 



Culture IVq +IVx.... 19° 19° 21° 36° 99° 



Culture IVx 19° 19° 23° 32° 96° 



Bacterial Counts. 

 In 48 hours. 



Culture IVq— 1,670,000,000. 

 Culture IVq + IVx— 1,380,000,000. 

 Culture IVx— 1 ,750,000,000. 

 44 



