EXPERIMENT STATION BULLETINS. 349 



Gelatin Stab. 



Uliform, becoming infundibuliform. 

 Agar. 



Glistening, opaque. 

 Bouillon . 



Turbid, with scum and sediment. 

 Potato. 



Moist, discolored. 

 Milk. 



Digested. Also see ciiart combination IVg and IVx. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). None. 

 Proteolysis . 



Gelatin liquefied. Casein digested. 

 Biochemical tests. 



No indol, hydrogen sulphide, or ammonia. Nitrates reduced. 

 Chromogenesis. 



No pigment. 



IVh. 

 Morphology. Bacillus . 



Size — average diamcfor, 1 micron; length, 2.2 microns to 4.5 microns. 



Motility — motile. 



Staining — stains readily. 

 Gelatin Colony. 



Round, entire, finely granular. 

 Gelatin Stab. ' 



Filifonn, becoming crateriform. 

 Agar. 



Glistening, opaque. 

 Bouillon. 



Turbid with sediment. 

 Potato. 



Moist, abundant growth. 

 Milk. 



Litmus changed to green. Also see chart combination IVh and IV.v. 

 Fermentation ol' Sugars (Dextrose, Lactose, Saccharose). None. 

 Proteolysis. 



Gelatin lic[ucfied. Casein not noted. 

 Biochemical tests. 



No indol, hydrogen sulphide, or ammonia. Nitrates reduced. 

 Chromogenesis. 



Green. 



IVi. 



Morphology. Bacillus. 



Size — Average diameter, .9 microns; length, 2.2 to 3 microns. 



Motility — motile. 



Staining — stains readily. 

 Gelatin Colony. 



Not noted. 

 Gelatin Stab. 



Infundibuliform . 

 Agar. 



Dull, opaque. 

 Bouillon. 



Turbid, witli sediment. 

 Potato. 



Abundant, discolored growtii. 

 Milk. 



Loppered, fum curd. 

 Fermentation of Sugars (Dextrose, Lactose, Saccharose). Dextrose and Saccharose 



fermented; I^actose not fermented. 

 Proteolysis. 



Gelatin liquefied. 

 Biochemical tests. 



No indol, or ammonia; hydrogen sulphide present. Nitrates are reduced. 

 Chromogenesis . 



No pigment. 



