EXPERIMENT STATION BULLETINS. 



355 



Culture Ig irioiints from 18"^ acid to 30° in sixty-seven hours, as rapidly as 

 the check lactic culture, and yet the acidity of Ig will not account for the 

 increased acidity of Ig + Ij. Now again, if the acidity of IIj were added 

 to the acidity of IIx in forty-eight houi-s, perhaps the increased acidity of 

 IIj 4- IIx over IIx would be understood. 



These three possibilities, therefore, exist in accounting for the increased 

 development of the lactic micro-organisms: 



First, where the milk does not become acid by the cultivation of the as- 

 sociate germ. 



Second, where the milk becomes acid by the gro^viih of the associate micro- 

 organism, but this acidity cannot account for the increased acidity of the 

 combined culture. 



Third, where the milk becomes acid by the growth of the associate micro- 

 organisms, and this added acidity may account for the increased acidity 

 of the combined culture. 



To support these contentions let us bring together into groups the sub- 

 stantiating facts for each. 



Time after uioculation. lirs. 



Culture la 18° 



Culture la + Ij 18° 



Culture Ij 18° 



Time after inoculation. hrs. 



Culture Ih 16° 



Culture Ih + Ij 16° 



Culture Ij 16° 



Time after inoculation. hrs. 



Culture He 18° 



Culture He + IIx 18° 



Culture IIx 18° 



Time after inoculation. hra. 



Culture Ilg 21° 



Culture Ilg + IIx 21° 



Culture IIx 21° 



Time after inoculation. hrs. 



Culture Ilia 16° 



Culture Ilia + Illx 16° 



Culture IIIx 16° 



Time after inoculation. hrs. 



Culture Illb 14° 



Culture Illb + IIIx 14° 



Culture IIIx 14° 



Time after inoculation. hrs. 



Culture Hie 14° 



Culture IIIc + lllx 14° 



Culture IIIx 14° 



Time after inoculation. hrs. 



Culture Illi 15° 



Culture Illi + IIIx 15° 



Culture IIIx 15° 



Time after inoculation. hr.<i. 



Culture IVh 14° 



Culture IVh + IVx 14° 



Culture IVx 14° 



Time after inoculation. hrs. 



Culture IVi 15° 



Culture IVj + IVx 15° 



Culture IVx 15° 



Time after inoculation. hrs. 



Culture IVI 18° 



Culture' IVI + IVx 18° 



