382 . . STATE BOARD OF AGRICULTURE. 



SERIES VII. 



In t^eries No. YIL, we Lave attempted to duplicate Stoklasa's work 

 by using the nutrient solution which he recommends and which has the 

 following composition : 



Distilled water 1000 c. c. 



Potassium sulphate .' 1.0 gram 



Magnesium chloride 0.5 " 



Ferrous sulphate 0.1 " 



100 cubic centimeters of this stock solution were diluted with 800 c. c. 

 of distilled water for each flask before use. 



From the results shown in tables Xos. 4. .5, G, 7, 8, 9, it will be seen 

 that we have been unable to obtain anything like the quantities of 

 phosphoric acid reported by Stoklasa. This has led us to follow up his 

 work and repeat his tlask experiments, using his special medium, the 

 same organisms except for a possible dillerence in vigor due to attenua- 

 tion, but different bone meal. At* the end of 3-3 days, determinations 

 were made for soluble phosphoric acid but still we were unable even 

 to approach his results. The largest amount of phosphoric acid ren- 

 dered available in any of our experiments thus far cited was 1.19% PaO-. 

 by germ No. 8 in Series I. while the minimum quantity secured by 

 Stoklasa was 9.19% by B. fluorescens liquefaciens. From this we must 

 conclude that under the conditions of the experiment, the bone, being 

 the only variable, must have been responsible for the difference in re- 

 sults. Our determinations duplicating Stoklasa are given in Table No. 

 10. 



