EXPERIMENT STATION BULLETINS. 609 



Only a slight reaction developed. The sexual hairs developed no matting 

 or staining. The dam of calf 997 A was two years old at the time of 

 the parturition. The milk and blood of the dam gave a negative reaction 

 before and after parturition. 



Heifer calf 1000 A, Table 5, was fed upon naturally infected milk for 

 a period of fourteen weeks. The blood was negative before feeding the 

 milk, and continued to remain negative throughout the period of feed- 

 ing. No matting of the sexual hairs below the vulva was observed. The 

 dam of calf 1000 A was two years old at the time of this parturition. 

 The blood and milk of the dam were negative before and after parturi- 

 tion. 



Bull calf 1001 A, Table 6, was fed upon naturally infected milk for 

 a period of five weeks. The blood of the calf gave a positive reaction 

 before feeding. The positive reaction persisted for three weeks. The 

 sexual hairs around the sheath became slightly matted the second week 

 of feeding. A microscopical examination of the sediment collected from 

 the matted hairs revealed only bacteria, uric acid crj'stals and epithelial 

 cells. The dam of calf 1001 A was seven years old at the time of this 

 parturition. The cow has never aborted or had an abnormal parturition. 

 The blood was negative and the milk was positive after parturition. 



Bull calf 1002 A, Table 7, was fed upon non-infected milk for the 

 first week after birth and then fed upon naturally infected milk for 

 thirteen weeks. The blood was negative before feeding milk and con- 

 tinued to remain negative throughout the period of feeding. The sexual 

 hairs about the sheath developed a slight matting and brownish black 

 staining in the fourth week. The matting was not consistent. The 

 hairs would mat together after urination, but would separate again on 

 drying. Several microscopic examinations were made of the washings 

 from the sexual hairs. No pus cells were found. The dam of calf 1002 A 

 was five years old at the time of this parturition. The cow has never 

 aborted or had an abnormal parturition. The blood and milk of the 

 cow were negative before and after parturition. 



Bull calf 100 A, Table 8, was allowed to suckle its mother for three 

 days after birth and then separated. The calf has since been receiving 

 naturally infected milk. The calf was bled three days after birth at 

 which time a positive reaction was obtained. The positive reaction per- 

 sists at this writing. The sexual hairs have remained unmatted and 

 unstained. The dam of calf 100 A was three years old at the time of 

 this parturition. The calf of the first pregnancy was aborted. The 

 blood and milk of the cow Avere positive after parturition. 



Bull calf 101 A, Table 9, was allowed to suckle its mother for three 

 days after birth at which time it was separated and fed upon naturally 

 infected milk. The blood of the calf has repeatedly given a negative 

 reaction. The sexual hairs have remained unmatted and unstained. 

 The dam of calf 101 A was two years old at the time of this parturition. 

 The blood and milk of the cow gave a negative reaction before and after 

 parturition. 



Heifer calf 102 A, Table 10, was fed upon naturally infected milk for 



a period of fifteen weeks. The blood of the calf was negative before 



feeding milk. No test was made in the first week after feeding was begun. 



Positive agglutination and complement fixatioij ri^i^tions were obtained 



77 



