610 STATE BOARD OF AGRICULTURE. 



in the second week. A slight reaction persisted for six weeks. Succeed- 

 ing tests since the sixth week have been negative. No matting of the 

 sexual hairs below the vulva occurred during the feeding of milk. The 

 dam of calf 102 A was three j-ears old at tlie time of this parturition. 

 The cow has never aborted or had an abnormal parturition. The cow was 

 injected subcutaneously with five cubic centimeters and ten cubic centi- 

 meters of a killed suspension and twenty c.c. of a live suspension of 

 Bad. abortus at ten day intervals during the seventh month of gesta- 

 tion. The blood of the cow was positive to the agglutination and com- 

 plement fixation tests at the time of parturition. The milk was also 

 positive to both tests. 



Heifer calf 103 A, Table 11, was fed upon naturally infected milk for 

 six weeks. Complement fixing antibodies were present in the blood of 

 the calf a few hours after birth. Agglutinins appeared in the fourth 

 week. The positive reaction persisted for six weeks. The calf died 

 in the seventh week of white scours. No matting of the sexual hairs 

 below the vulva was observed. The dam of calf 103 A was three years 

 old at the time of this parturition. This parturition was premature, 

 being about two weeks before time. The dam was treated during the 

 eighth month of gestation, in the same manner as dam 102. The blood 

 and milk of the cow contained agglutinating and complement fixing 

 bodies at the time of the parturition. 



Bull calf 104 A has been receiving naturally infected milk for thirteen 

 weeks. The blood lias never given a positive reaction to the agglutina- 

 tion or complement fixation tests. The sexual hairs around the prepuce 

 have never shown any matting or staining. The dam of calf 104 A was 

 two years old at the time of this parturition. The blood and milk of 

 the cow have never given a positive reaction to the agglutination or 

 complement fixation tests. 



In order to compare a possible blood reaction due to a naturally in- 

 fected milk with that due to an artificially infected milk, a calf was 

 fed upon non-infected milk plus a culture of Bad. abortus. 



Bull calf 1003 A, Table 13, was fed upon non-infected milk plus five 

 cubic centimeters of a fortj^-eight hour bouillon culture of Bad. abortus 

 (the culture being added to the milk at each feeding) for a period of twelve 

 weeks. The blood of the calf was negative before feeding the mixture. 

 A high antibody index developed at the end of the first week of feeding. 

 The antibody index remained constant until the end of the fourth week. 

 At this time the antibodv index began to decrease. On the ninth week 

 the blood was negative and has remained negative up to the time of this 

 writing. A distinct matting and staining of the sexual hairs around 

 the sheath appeared in the twelfth week of feeding. A microscopic ex- 

 amination of the hairs revealed epithelial cells, uric acid crystals, and 

 many bacteria. No pus cells were found. The dam of calf 1003 A was 

 four years old at the time of this parturition. The calf of the second 

 pregnancj'^ was aborted. The blood and milk were negative before parturi- 

 tion. The cow died of milk fever shortly after parturition and conse- 

 quently no post parturition tests were made. 



In order to maintain a control on the naturally infected milk fed to 

 calves, the infected milk was pasteurized at a temperature of 63 °C. for 

 fifteen minutes. The milk was then fed to a calf. A temperature of 



