626 STATE BOARD OF AGRICULTURE. 



A STUDY OF THE PRESENCE OF BACTERIUM ABORTUS (BANG) 



IN MILK. 



BY L. H. COOLEDGE. 



INTRODUCTION. 



Part I. 



Methods of studying the presence of Bad. ahortus (Bang) in Milk. 

 Introduction. 

 Review of literature. 

 Technic emploj^ed. 



Complement-fixation test. 

 Agglutination test. 

 Investigational work. 



Comparing the Bad. ahortus antibed}^ content of the blood with 



that of the milk. 

 Comparing the antibody content of the milk from quarter to 



quarter. 

 Comparing the agglutination and complement-fixation reactions 

 for Bad. ahortus infection, using milk instead of blood serum. 

 Studying the agglutinins in milk after the introduction into the 



cow's udder of pure culture of Bad. ahortus. 

 Studying the agglutination reaction of milk from cows with 



records of frequent abortions. 

 Studying the agglutination reaction of milk from cows that have 



never aborted. 

 Studying millv containing antibodies but not producing typical 



lesions in guinea-pigs. 

 Studying milk containing antibodies and producing typica^ 

 lesion in guinea-pigs. 



Part II. 



A study of the effect of Bad. ahortus upon man. 

 Introduction. 

 Review of literature. 

 Technic employed. 

 Investigational work. 

 A study of the presence of antibodies for Bad. ahortus in the 



blood of adults. 

 A study of the antibody content of the blood of persons drinking 



a naturally infected milk. 

 A study of the antibody content of human blood serum while in- 

 dividuals are drinking a milk which does not contain antibodies 

 but to which a pure culture of this organism is added. 



