EXPERIMENT STATION BULLETINS. 



641 



TABLE XI.— A COMPARISON OF THE ANTIBODY AND CELLULAR CONTENT OF THE 

 FORE MILK AND STRIPPINGS OF REACTING MILK CAPABLE OF CAUSING TYPICAL 

 INFECTIOUS ABORTION LESIONS IN GUINEA PIGS. 



(Agglutination reaction when various quantities of milk were added to test tubes containing bacterial 



suspension.) 



Cow No. 91: 

 Fore Milk . 

 Strippings . 



Cow No. 81: 

 Fore Milk. 

 Strippings . 



Cow No. 77: 

 Fore Milk. 

 Strippings . 



Cow No. 61: 

 Fore Milk . 

 Strippings . 



Cow No. 996: 

 Fore Milk . . 

 Strippings . . 



Right Rear Quarter. 



Agglutina- 

 tion. 



—t lO 

 O lO 



+ 



+ 



+ 



+ + 



+ ! + 

 + + 



+ 



Cells 

 per c. c. 



400,000 

 3,600,000 

 A. 



10,000,000 



7,000,000 



A. 



30,000 

 210,000 

 A. 



30,000 

 360,000 

 B. 



30,000 

 450,000 

 C. 



Right Front Quarter. 



Agglutina- 

 tion. 



+ 



+ 



+ + 

 + + 



t!^ 



Cells 

 per c. c. 



1,200,000 



1,200,000 



A. 



1,500,000 



6,000,000 



A. 



3,000,000 



5,000,000 



A. 



30,000 

 210,000 

 B. 



1,900,000 



18,000,000 



C. 



Left Rear Quarter. 



Agglutina- 

 tion. 



+ 



+ 



+ 



Cells 

 per c. c. 



900,000 

 900,000 

 A. 



5,700,000 



3,600,000 



A. 



2,000,000 



23,000,000 



A. 



30,000 

 960,000 

 B. 



30,000 

 90,000 



c. 



Left Front Quarter. 



Agglutina- 

 tion. 



Cells 

 per c. c. 



4,500,000 



9,000,000 



A. 



480,000 

 7,000,000 

 A. 



60,000 

 2,700,000 

 A. 



30,000 

 30,000 

 B. 



30,000 

 210,000 

 C. 



A. Guinea pigs inoculated with 5 c.c. of milk from each quarter developed typical Bad abortus 

 lesions and blood reactions. 



B. Guinea pigs inoculated with 5 c.c. of milk from each quarter developed typical Bact. abortus 

 lesions and blood reactions. Exception, guinea pig inoculated from L. F. Quarter which did not de- 

 velop lesions. 



C. Right front quarter of this cow's udder inoculated with a pure culture of Bact. abortus 3 days 

 previous to this test. See Table V. 



Here we see that in uearly every case the strippings have as high or 

 a higher antibody and cellular content than does the fore milk. It seems 

 to the writer that this is the condition which might be expected in case 

 of an active Bact. abortus infection. The more vigorous manipulation 

 of the udder during the stripping process tends to force the antibodies 

 and cells of infected udder tissue into the milk stream. This results in 

 the strippings having a high antibody and cellular content. In cow 996 

 of this table the udder was artificially infected with a culture of Bact. 

 abortus and it will be noticed that the comparative strength of the re- 

 actions in the fore milk and strippings is the same as in the other in- 

 stances in which the infection appeared naturally. 



In Table XII will be found a comparison of the antibody and cellular 

 content of fore milk and strippings of reacting milk not capable of caus- 

 ing typical Bact. ahortus lesions and blood reactions in guinea pigs 

 with the 5 c.c. of milk used for inoculation, 

 81 



