EXPERIMENT STATION BULLETINS. 



649 



TABLE VI.— SHOWING THAT LOW PASTEURIZATION TEMPERATURES WILL NOT DE- 

 STROY BACT. ABORTUS AGGLUTININS IN MILK AND CREAM. 



Agglutination Test. 



.1 

 c. c. 



.05 

 c.c. 



.025 

 c c. 



.01 .005 

 c.c. c.c. 



Whole raw milk . . . 



Held 60°C., 20 min 



30 min 



" 40 min 



Held 63°C., 20 min 

 30 min 

 40 min 



Held 65°C., 20 min 

 30 min 

 40 min 



Whole raw cream . . 



Held 60°C., 20 min 

 30 min 

 40 min 



Held 63°C., 20 min 



30 min 



" 40 min 



Held 65 C, 20 min 

 30 min 

 40 min 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 P 

 P 



+ 

 + 

 + 

 + 

 P 

 P 



+ 

 + 

 + 

 + 

 P 

 P 

 P 

 + 

 P 

 P 



+ 

 + 

 + 

 + 

 P 

 P 



+ 

 + 

 + 



+ 



+ 

 + 

 + 

 P 



In Table V will be found further tests showing the presence of anti- 

 bodies in the blood of adults drinking milk. Here again we see a tend- 

 ency for the antibodies to appear more regularly in the blood of those 

 drinking raw milk. In instances in which the antibodies appear in the 

 blood of those drinking pasteurized milk there is the possibility of their 

 being derived from the ingestion of other dairy products which are not 

 pasteurized or are pasteurized at a temperature not suflScient to destroy 

 the antibodies present. The individuals tested in Table V eat more or 

 less ice cream which is made from cream pasteurized at a temperature 

 of 60° C. for 30 minutes. That this temperature is not sufficient to 

 destroy the antibodies may be seen in Table VI. Experiments have 

 shown that a momentary temperature between 70° C. and 75° C. is 

 necessary to destroy the agglutinins. 



Part III. 



Facts Disclosed in a Study of the Presence of Bad. abortus in Milk by 



Means of the Agglutination Test. 



INTRODUCTION. 



Through the studies of other investigators and the results shown in 

 another part of this paper we know that Bad. adortus is often present 

 in the milk of apparently normal cows. It is also shown that the or- 

 ganism present in the cow's udder is in a virulent form capable of caus- 

 ing very pronounced lesions when five cubic centimeters of the milk are 

 injected intra-abdominally into a guinea pig. 



In an early part of this paper it is shown that this infection of the 

 udder may be detected by means of the so-called serum tests when 

 blood serum is replaced by milk as the substance to be tested. In this 



