EXPERIMENT STATION BULLETINS. 



305 



(liiatin tube-cult U7e. — A white granular growth appears along the whole line of 

 puncture with an irregular edge, apparently due to the fact that the colonies appear- 

 ing near the border of the puncture are not thick enougli to coalesce and have no 

 tendency to run together. At the surface of the gelatin, the colony spreads much as 

 a colony in the gelatin plate and corresponds almost identicallj^ with the colony illus- 

 trated in Fig. 8, with this exception, — it is denser and larger and bears the same 

 relation as a giant colony to a simple colony. Fig. 9 will illustrate the growth along 

 the pimcture at the end of twenty-four hours. Gas bubbles will be noticed deep in the 

 gelatin. 



Bouillon luhc-culture. — In bouillon the growth is heavy and soon settles to the 

 bottom in a white, curdy mass, leaving the supernatent bouillon clear. In a few days 

 a white, smooth, dense scum forms over the surface and projects about four milli- 

 meters up the side of the tube. 



Inclined af/ar tube-culture.— A heavy, pearly-white raised growth appears along the 

 line of inoculation. It has little tendency to spread over the surface and its border is 

 escalloped. 



Eftiitarch vegetable cultures — Potato. — A thick, creamy, gi-ayish-white growth cov- 

 ers the surface of the potato. As it grows older, it becomes slightly darker. The 

 odor is that of sour potatoes. It has no tendency to spread. On some potatoes the 

 growth is very limited and on others very luxuriant. This is doubtless due to the 

 varying reaction of the potatoes. 



Oyster plant. — This seems to be an especially favorable medium for the growth of 

 this germ. A heavy coating forms of a grayish-white color and extends over the 

 entire surface. Throughout the growth, numerous pin-holes are visible with a crater- 

 like ridge around them, which appear as if gas had escaped from the lower stratum. 



I 



Fig. ;i. 



Parsnip.— A very thin, moist film is perceptible on the surface. The parsnip retains 

 its natural color and the odor is that of the parsnip. 



Rutabaga.— On this vegetable the growth is white and moist, but it is limited to 

 39 



