328 STATE BOARD OF AGRICULTURE. 



indicative of the disease. This led to tho cstablit^liinent ot the bacteriological work in 

 connection with the e.vpcrinient station. 



Early in the year of 18!lU the attention of the Board was called to the use of 

 tuberculin as a test in diagnosing this disease. The Board placed the matter in the 

 hands of President Snyder, Director Smith and Dr. Grange. These gentlemen unani- 

 mously agreed to apply the test to the College lierd. This was done by Dr. Grange 

 March lil and April 1, 18!)(i. Nine animals out of fifly-scven responded. Besides tliese. 

 there were some which gave suspicious reactions. These were again tested on May 11 

 and 12 of the same j'ear. Two of them gave distinct reactions. Again on August 

 19 and 20 Dr. Grange applied tlie tuberculin tost to those still holding out as 

 suspicious and found one reacting. In April of this year the Board granted Dr. 

 Grange permission to hold these reacting animals for experimental purposes. On 

 June 30 the Director of the Station communicated to the Board the general outline of 

 experiments with this disease which has since been followed. In the spring of 1897, 

 April 20 and 21, anotiier test was applied to the whole herd, including the animals 

 already condemned, by Dr. Grange. Five animals at this time responded to the 

 test, in addition to those having already reacted. Septejuber G and 7, 1897, the 

 author tested fifteen graded cows which had been purchased by the College for dairy 

 animals. None responded to the test. October 13 and 14 1 applied the test to fifteen 

 more grade cows bought for the same purpose as the others, and fovmd none reacting. 

 At the same time, the test was applied to thirteen sus])ects remaining over and 

 two reacted. March 31 and April 1, 1898, the annual test was again made of the 

 whole herd and only one animal reacted in addition to those already condemned. 

 When the annual test was applied in 1899, March 30 and 31, to the regular herd, 

 one additional animal responded. Since this time there has been no test applied to 

 all the animals. 



The details of the test of each animal reacting, together with other interesting data, 

 will be given under the history of the respective animals. 



ROSA BONHEUR 5, 11227. 



This cow had for her sire Meadow Brook Chief, 1909, and her dam Rosa Bonheur. 

 890, H. H. B., and was born March 20, 1888. Her breed is Holstein. She first reacted 

 .\pril 1, 1896. Her reactions at that time and since are as follows: 



March 31, 1896, before injection, 99.0, 100.6, 100.8. 101.2, 100.8. 



April 1. 1896, after injection. 100.2, 100.4, 100.6. 100.1. 101.6. 102.9, 103.3, 102.6, 

 102.6. 



August 26, 1896, before injection, 99.0, 101.4, 101.8, 102.1, 101.6. 



August 27, 1896, after injection, 101.6, 101.8, 101.6, 101.8, 102.1, 102.8, 104.0, 105.0, 

 104.4. 



April 22, 1897, before injection, 101.5, 101.1, 101.0, 101.3, 101.4. 



April 23, 1897, after injection, 101.0, 101.2, 101.2, 101.9, 101.1, 101.4, 104.6, 103.2, 

 102.8. 



October 13, 1897, before injection, 100.5, lOl.G, 101.3, 100.8, 101.7. 101.3. 102.2. 



October 14, 1897, after injection, 101.2, 101.2. 101.4, 101.1, 101.1, 101.4, 102.0, 101.8. 



101.5, 102.5, 102.3. 



March 31, 1898, before injection, 99.7, 99.3, 97.7, 97.1. 97.2, 98.4, 97.9, 99.0, 98.6, 98.4. 



April 1, 1898, after injection, 97.7, 99.3, 99.8, 98.9. 99.2, 96.9. 98.3, 96.7, 98.4, 97.8, 

 98.3, 98.0. 



October 14, 1898. before injection, 100.2, 100.6, 99.0. 98.6, 100.0, 99.9, 100.4, 101.0. 

 1»9.8, 101.4. 



October 15, 1898, after injection, 100.8, 99.0, 99.3, 100.0, 99.4, 99.0, 97.2, 99.7, 100.7. 

 100.0. 



March 30, 1899, before injection, 97.8, 97.0, 98.5, 98.5, 99.3, 98.4, 97.4, 97.8, 97.8, 97.3. 



iVIarch 31, 1899, after injection, 96.8, 98.9, 99.1, 100.9, 97.8, 97.4, 98.5, 98.4. 97.9. 

 99.2, 97.8, 97.0. 



October 9, 1899, before injection, 99.5, 98.6, 100.0, 99.9. 100.2, 100.8, 99.8, 101.5, 

 101.4, 100.8. 



October 10, 1899, after injection, 99.7, 99.6, 100.0, 100.3. 99.8, 100.1, 100.8, 100.4. 



100.6, 100.5, 101.2. 



It will be seen from these reactions that this cow did not respond to the tuberculin 

 test after April, 1897. 



At no time did her excreta or milk show the presence of tubercle bacilli. I was 

 never able to secure sputum or nostril discharges which contained tubercle bacilli. 



